Team:Newcastle/27 July 2010

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(Preparation for cloning of the rocF BioBrick)
 
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{{Team:Newcastle/mainbanner}}
{{Team:Newcastle/mainbanner}}
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===Aims of genomic DNA extraction experiment===
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=Genomic DNA extraction experiment=
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The aim of today's experiment is to extract genomic DNA from ''Bacillus subtilis'' strain 3610 and 168,genes from which will be needed for the swarming biobrick and ''rocF'' biobrick.
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====Procedure====
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[[Image:Newcastle alan chromosome.jpg|thumb|200px|right]]
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* Please refer to [[Team:Newcastle/DNA extraction| DNA extraction of ''Bacillus subtilis'']]
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[[Image:Newcastle ice chromosome.jpg|thumb|200px|right]]
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==Aims==
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The aim of today's experiment is to extract genomic DNA from both ''B. subtilis'' strains 168 and 3610. The genes necessary for the [[Team:Newcastle/Swarming|swarming BioBrick]] and [[Team:Newcastle/Urease|''rocF'' BioBrick]] will then hopefully be obtained from the genomic DNA using PCR.
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====Discussion====
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==Protocol==
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At the end of the DNA precipitation step, we did observe a small white pellet in all the eppendorf tubes.
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* Please refer to: [[Team:Newcastle/DNA extraction| DNA extraction of ''B. subtilis'']] for materials required and protocol.
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====Conclusion====
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==Discussion==
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Th experiment was successful and we would check the content and the purity of the extracted DNA by using PCR on 28th July, 2010.
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At the end of the DNA precipitation step, we observed a small white pellet in all the eppendorf tubes.
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==='''Gel Electrophoresis'''===
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==Conclusion==
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The experiment was a success! The quality of the extracted DNA will be checked by using PCR on 28th July, 2010.
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=Preparation for cloning of the rocF BioBrick=
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====Protocol====
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==Results==
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* Please refer to [[Team:Newcastle/Gel electrophoresis|Gel Electrophoresis]]
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Yesterday, we transformed ''E. coli'' DH5α with pSB1C3 and [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] with [http://partsregistry.org/Part:BBa_B0014 BBa_B0014]. After checking the plates today for colonies we observed lots of pink colonies on the pSB1C3 plates and numerous white colonies on the [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] with [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] plates. Plates were then stored at 4°C as colonies will be used for minipreps.
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=====Materials=====
 
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# 50ml of 1% Agarose TAE
 
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# 5µl SafeView
 
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# TAE Buffer
 
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====Discussion====
 
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Latest revision as of 22:12, 27 October 2010

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Contents

Genomic DNA extraction experiment

Newcastle alan chromosome.jpg
Newcastle ice chromosome.jpg

Aims

The aim of today's experiment is to extract genomic DNA from both B. subtilis strains 168 and 3610. The genes necessary for the swarming BioBrick and rocF BioBrick will then hopefully be obtained from the genomic DNA using PCR.

Protocol

Discussion

At the end of the DNA precipitation step, we observed a small white pellet in all the eppendorf tubes.

Conclusion

The experiment was a success! The quality of the extracted DNA will be checked by using PCR on 28th July, 2010.

Preparation for cloning of the rocF BioBrick

Results

Yesterday, we transformed E. coli DH5α with pSB1C3 and [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] with [http://partsregistry.org/Part:BBa_B0014 BBa_B0014]. After checking the plates today for colonies we observed lots of pink colonies on the pSB1C3 plates and numerous white colonies on the [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] with [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] plates. Plates were then stored at 4°C as colonies will be used for minipreps.

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