Latest revision as of 18:51, 23 July 2010

14 July 2010

Run a gel for the PCR of the previous day -> worked
Purification of PCR’s product -> ok
Preparation of the BBα_151020 (streak out)
Competence Calculation of E.Coli DB3.1 (+pUC10, Amp) -> ok
Look at Asaia with microscope -> we have cells (pictures)
Growth Curve for Asaia (OD measurement each hour) -> both manually (ok) + with the plate reader
Plated Asaia to recover some WT (streak out)
Preparation of Asaia Culture (for Lemaître experiment) with Kan
Protocol LateX template
O/N culture of the E.coli DH5 transformed with the BB Antibiotics resistance (4x) -> miniprep
Learning HTML for the wiki
Text for the project presentation due to iGem
Writing Protocols of basic procedures for the wiki page
Culture of Asaia + Kan

@@ Line 4: / Line 4: @@
 = 14 July 2010 =
-Empty yet but it will be filled soon, don't worry ;)
+*Run a gel for the PCR of the previous day -> worked
+*Purification of PCR’s product -> ok
+*Preparation of the BBα_151020 (streak out)
+*Competence Calculation of E.Coli DB3.1 (+pUC10, Amp) -> ok
+*Look at Asaia with microscope -> we have cells (pictures)
+*Growth Curve for Asaia (OD measurement each hour) -> both manually (ok) + with the plate reader
+*Plated Asaia to recover some WT (streak out)
+*Preparation of Asaia Culture (for Lemaître experiment) with Kan
+*Protocol LateX template
+*O/N culture of the E.coli DH5 transformed with the BB Antibiotics resistance (4x) -> miniprep
+*Learning HTML for the wiki
+*Text for the project presentation due to iGem
+*Writing Protocols of basic procedures for the wiki page
+*Culture of Asaia + Kan
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