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TeamNewcastleNanoDrop Spectrophotometer
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| + | {{Team:Newcastle/mainbanner}} | ||
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| + | =NanoDrop Spectrophotometer= | ||
[[Image:Newcastle_nanodrop_2.jpeg|thumb|350px]] | [[Image:Newcastle_nanodrop_2.jpeg|thumb|350px]] | ||
[[Image:Newcastle_nanodrop_1.jpeg|thumb|350px]] | [[Image:Newcastle_nanodrop_1.jpeg|thumb|350px]] | ||
[[Image:Newcastle_nanodrop_3.jpeg|thumb|350px]] | [[Image:Newcastle_nanodrop_3.jpeg|thumb|350px]] | ||
| - | Nanodrop | + | Nanodrop uses absorbance to measure the concentration and purity of DNA, RNA and protein. |
| + | The ideal concentration of DNA is 150 ng/ml. | ||
| - | + | ==Materials required== | |
| + | * Pipettes | ||
| + | * Nanodrop machine | ||
| + | * Appropriate blanking solution | ||
| + | * Appropriate samples | ||
| - | + | ==Procedures== | |
| - | + | ||
| - | == | + | |
# Log onto computer and select Nanodrop program from the desktop (ND 1000) | # Log onto computer and select Nanodrop program from the desktop (ND 1000) | ||
| - | # | + | # Wipe the pedestal and top of the Nanodrop machine with a tissue. Place 3 µl of water to nib of pedestal and press blank to clean.[[Image:drop3.jpg|150px]] [[Image:drop.jpg|150px]] [[Image:drop2.jpg|150px]] |
| - | # | + | # After blanking, wipe the water off and equalize the Nanodrop using 3 μl of the appropriate buffer used to resuspend the sample. (example: Miniprep samples should be equalized with EB buffer). |
| + | # Use DNA-50 for DNA samples. | ||
# Wipe to remove buffer and apply 3 μl of sample to nib. Press measure. | # Wipe to remove buffer and apply 3 μl of sample to nib. Press measure. | ||
# If dealing with multiple samples, clean the equipment with water at regular intervals (about every 10 samples) | # If dealing with multiple samples, clean the equipment with water at regular intervals (about every 10 samples) | ||
# After measurements, clean the equipment with 3 μl of water on the spectrometer and press blank. Wipe and log off. | # After measurements, clean the equipment with 3 μl of water on the spectrometer and press blank. Wipe and log off. | ||
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| + | '''Go back to our [[Team:Newcastle/Protocol list|Protocol List]]''' | ||
| + | |||
| + | {{Team:Newcastle/footer}} | ||
Latest revision as of 10:26, 13 August 2010
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NanoDrop Spectrophotometer
Nanodrop uses absorbance to measure the concentration and purity of DNA, RNA and protein. The ideal concentration of DNA is 150 ng/ml.
Materials required
- Pipettes
- Nanodrop machine
- Appropriate blanking solution
- Appropriate samples
Procedures
- Log onto computer and select Nanodrop program from the desktop (ND 1000)
- Wipe the pedestal and top of the Nanodrop machine with a tissue. Place 3 µl of water to nib of pedestal and press blank to clean.
- After blanking, wipe the water off and equalize the Nanodrop using 3 μl of the appropriate buffer used to resuspend the sample. (example: Miniprep samples should be equalized with EB buffer).
- Use DNA-50 for DNA samples.
- Wipe to remove buffer and apply 3 μl of sample to nib. Press measure.
- If dealing with multiple samples, clean the equipment with water at regular intervals (about every 10 samples)
- After measurements, clean the equipment with 3 μl of water on the spectrometer and press blank. Wipe and log off.
Go back to our Protocol List
   
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