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Team:METU Turkey/Planning
From 2010.igem.org
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<div align="center" style="margin:15px 0px 0px 0px"><img style="border: 0px solid ; width: 950px; height: 300px;" alt="w7" src="http://partsregistry.org/wiki/images/d/d0/Schedule.JPG"></a></div> | <div align="center" style="margin:15px 0px 0px 0px"><img style="border: 0px solid ; width: 950px; height: 300px;" alt="w7" src="http://partsregistry.org/wiki/images/d/d0/Schedule.JPG"></a></div> | ||
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<br><h2>PLANNING</h2> | <br><h2>PLANNING</h2> | ||
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| - | <br>Cloning | + | <br><h3>Cloning</h3> |
<br> - Design and ordering of DNA parts | <br> - Design and ordering of DNA parts | ||
<br> - Preparation of competent cells | <br> - Preparation of competent cells | ||
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| - | <br>Cell Sensor Experiments | + | <br><h3>Cell Sensor Experiments</h3> |
<br> - Fermentor studies | <br> - Fermentor studies | ||
<br> - Flask experiments | <br> - Flask experiments | ||
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| - | <br>CooA Expression and Purification | + | <br><h3>CooA Expression and Purification</h3> |
<br> - Overexpression optimization | <br> - Overexpression optimization | ||
<br> - Purification optimization | <br> - Purification optimization | ||
<br> | <br> | ||
| - | <br>Characterization: Isothermal Titration Calorimetry (ITC) | + | <br><h3>Characterization: Isothermal Titration Calorimetry (ITC)</h3> |
<br> - DNA component preparation | <br> - DNA component preparation | ||
<br> - Optimization studies | <br> - Optimization studies | ||
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| - | <br>Characterization: Electrophoretic Mobility Shift Assay (EMSA) | + | <br><h3>Characterization: Electrophoretic Mobility Shift Assay (EMSA)</h3> |
<br> - Optimization studies | <br> - Optimization studies | ||
<br> - Determination of Response Element - Transcription Factor Affinities | <br> - Determination of Response Element - Transcription Factor Affinities | ||
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| - | <br>Characterization: Intrinsic Tryptophan Fluorescence (ITF) | + | <br><h3>Characterization: Intrinsic Tryptophan Fluorescence (ITF)</h3> |
<br> - Optimization studies | <br> - Optimization studies | ||
<br> - Determination of Response Element - Transcription Factor Affinities | <br> - Determination of Response Element - Transcription Factor Affinities | ||
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Latest revision as of 03:33, 28 October 2010
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PLANNING
Cloning
- Design and ordering of DNA parts
- Preparation of competent cells
- Cloning
- Construction of Cell Sensor modules
- Validation
- Assembly of final construct
Cell Sensor Experiments
- Fermentor studies
- Flask experiments
- Solid culture experiments
- Myoglobin assay optimization
- Signal quantification with fluorescence spectroscopy
- Imaging with confocal laser scanning microscopy
CooA Expression and Purification
- Overexpression optimization
- Purification optimization
Characterization: Isothermal Titration Calorimetry (ITC)
- DNA component preparation
- Optimization studies
- Determination of Response Element - Transcription Factor Affinities
Characterization: Electrophoretic Mobility Shift Assay (EMSA)
- Optimization studies
- Determination of Response Element - Transcription Factor Affinities
Characterization: Intrinsic Tryptophan Fluorescence (ITF)
- Optimization studies
- Determination of Response Element - Transcription Factor Affinities
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