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| | <a href="https://static.igem.org/mediawiki/2010/b/b0/IC_PyrD.JPG"><img src="https://static.igem.org/mediawiki/2010/b/b0/IC_PyrD.JPG" width="200"/></a> | | <a href="https://static.igem.org/mediawiki/2010/b/b0/IC_PyrD.JPG"><img src="https://static.igem.org/mediawiki/2010/b/b0/IC_PyrD.JPG" width="200"/></a> |
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| | <a href="https://static.igem.org/mediawiki/2010/c/cb/IC_Assay_3_sept.jpg"><img src="https://static.igem.org/mediawiki/2010/c/cb/IC_Assay_3_sept.jpg" width="200"/></a> | | <a href="https://static.igem.org/mediawiki/2010/c/cb/IC_Assay_3_sept.jpg"><img src="https://static.igem.org/mediawiki/2010/c/cb/IC_Assay_3_sept.jpg" width="200"/></a> |
| The Team
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| The team with advisors Chris and Tom
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| The Imperial College iGEM Team
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| Some team members with SynBio icon Jay Keasling!!!
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| Maddie and a bit of Kirill in labs
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| Maddie in labs again
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| Nick
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| Maddie in labs... AGAIN
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| Kirill, Maddie and Nick
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| Nick (posing?)
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| Piotr focusing, Anita not so much!
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| Piotr and Anita
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| Maddie and Kirill
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| Anita and Ben
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| Nick knows ONE song on the guitar! Wow
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| Ania, Kyasha and Florian
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| The team and some of the advisors at Eastside.
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| Kyasha, Maddie and Harriet
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| Some of the team members at Eastside!
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| The team at the Human Practices Panel. Don't we look excited???
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| Our lovely advisors at the Human Practices Panel.
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| Some members of the team
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| Flo and Harriet
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| Susanna criticising our ideas?
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| Harriet and Wolf
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| Flo =)
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| Our advisors
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| Ben...
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| Wet Lab
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Surface Protein
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| Surface Protein Team Logo
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| Assembly Strategy for LytC
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| Gel confirming that DNA (pSB1C3 and pSB1AK3 with BOO14) has been digested properly by PstI and EcoRI.
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| Gel of PCR confirming pSB1C3 has been amplified.
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| Picture estimating the concentration of purified BOO14 and pSB1C3.
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| Colony PCR to detect sucessfully transformed bacteria.
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| 3 PCR reactions to determine the most effective protocol to amplify the CWB.
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| Gel of a diagnostic digest of the vector confirming that the terminator has been inserted.
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| Isolation of the promoter pVEG by gel purification.
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| Determining the ratio of pSB1C3 to pVEG for ligation.
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| Determining the ratio of lytC to pSB1C3 for ligation.
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Vectors
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| Vectors Team Logo
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| Assembly strategy for the AmyE vector
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| Assembly strategy for the PyrD vector
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| K14002 and oligo assembly
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| K143070 and K11064 assembly step
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| LacI Testing Vector
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XylE
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| XylE Team Logo
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| Team XylE
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| The J23101 gene in a biobrick vector containg RFP gene.
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| Midi-prep XylE digestion with xbaI and PstI
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| Gel analysis of XylE, J23101 promoter and pSB1C3 vector samples
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| Catechol assay (before)
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.jpg)
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| Catechol assay (after) - Can you spot the difference?
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| Catechol assay on XylE-trasformed cells in a 96-well plate
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| First Successful Catechol Assay!
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| First Successful Catechol Assay!
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| :)
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| Test confirming that yellow product of XylE enzmymatic reaction is leaks back from the cytosol into the solution.
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| The coloured pigment is transported out of the cells.
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| Spectra of XylE transformed E.coli after addition of catechol assay
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| Catechol Assay cuvette setup
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| Catechol Assay cuvette setup
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| Human Practices
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| Human Practices panel: advisors
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| The team looks reeeeaaaalllly interested
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| Maddie's notes from the HP panel
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| Notes...
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| Notes...
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| Should scientists be allowed to create artificial life?
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| Should scientists be allowed to create artificial life?
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| Building prototypes informed our design
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| We wanted our kit to be as useful as possible
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| This is the first design...
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| A prototype of our dipstick concept!! So cool!!!
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| More pictures of our awesome prototype!!!
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"
