Team:Edinburgh/Notebook/Green light sensor
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<li><a href="https://2010.igem.org/Team:Edinburgh/BioBricks#Genomic">submitted parts</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/BioBricks#Genomic">submitted parts</a></li> | ||
<li><a href="https://2010.igem.org/Team:Edinburgh/Results#Genomic">results</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Genomic">results</a></li> | ||
- | <li><a href="https://2010.igem.org/Team:Edinburgh/Project/Future">future | + | <li><a href="https://2010.igem.org/Team:Edinburgh/Project/Future">the future</a></li> |
<li><a href="https://2010.igem.org/Team:Edinburgh/Project/References">references</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Project/References">references</a></li> | ||
</ul> | </ul> | ||
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<li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial" class="dir">bacterial BRIDGEs</a> | <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial" class="dir">bacterial BRIDGEs</a> | ||
<ul> | <ul> | ||
- | <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Core_repressilator">the | + | <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Core_repressilator">the project</a></li> |
<li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Red_light_producer">red light</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Red_light_producer">red light</a></li> | ||
<li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Red_light_sensor">red sensor</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Red_light_sensor">red sensor</a></li> | ||
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<li><a href="https://2010.igem.org/Team:Edinburgh/BioBricks#Bacterial">submitted parts</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/BioBricks#Bacterial">submitted parts</a></li> | ||
<li><a href="https://2010.igem.org/Team:Edinburgh/Results#Bacterial">results</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Bacterial">results</a></li> | ||
- | <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Future">future | + | <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Future">the future</a></li> |
<li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/References">references</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/References">references</a></li> | ||
</ul> | </ul> | ||
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<li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Tools">tools</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Tools">tools</a></li> | ||
<li><a href="https://2010.igem.org/Team:Edinburgh/Results#Modelling">results</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Modelling">results</a></li> | ||
- | <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Future">future | + | <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Future">the future</a></li> |
<li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/References">references</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/References">references</a></li> | ||
</ul> | </ul> | ||
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<li><a href="https://2010.igem.org/Team:Edinburgh/Human" class="dir">human BRIDGEs</a> | <li><a href="https://2010.igem.org/Team:Edinburgh/Human" class="dir">human BRIDGEs</a> | ||
<ul> | <ul> | ||
- | |||
<li><a href="https://2010.igem.org/Team:Edinburgh/Human/Communication">communication of science</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Communication">communication of science</a></li> | ||
- | <li><a href="https://2010.igem.org/Team:Edinburgh/Human/ | + | <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Branding">iGEM survey</a></li> |
- | + | ||
<li><a href="https://2010.igem.org/Team:Edinburgh/Human/Conversations">conversations</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Conversations">conversations</a></li> | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
<li><a href="https://2010.igem.org/Team:Edinburgh/Human/Epic">the epic</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Epic">the epic</a></li> | ||
- | <li><a href="https://2010.igem.org/Team:Edinburgh/ | + | <li><a href="https://2010.igem.org/Team:Edinburgh/Human/FutureApps">future applications</a></li> |
- | <li><a href="https://2010.igem.org/Team:Edinburgh/Human | + | <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Human">further thoughts</a></li> |
<li><a href="https://2010.igem.org/Team:Edinburgh/Human/References">references</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Human/References">references</a></li> | ||
</ul> | </ul> |
Latest revision as of 02:12, 28 October 2010
These notes correspond to the part of the project detailed here.
Green Light Sensor
8/10/2010
- running purified PCR: marker (lane 1), PhOR (Lane 2), Ccas (Lane 3).
19/10/20010
- Lane 1: marker
- Lane 2: PheA (very faint band or primer smear?)
- Lane 3: Ccas + PhoR ligation
22/10/2010
- PhoA + LacZ
23/10/2010
- Lane 1: marker
- Lane 2: phA promoter + lac Z
- rest- irrelevant
25/10/2010
- pCB + green light sensor
26/10/2010
- total green light sensor + reporter
27/10/2010
- green light sensor ligations into PSB1C3