Team:METU Turkey/Planning

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                       <a href="https://2010.igem.org/Team:METU_Turkey/Conclusion">Conclusions</a> |
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                       <a href="https://2010.igem.org/Team:METU_Turkey/Conclusions">Conclusions</a> |
                       <a href="https://2010.igem.org/Team:METU_Turkey/Future_Work">Future Work</a> |
                       <a href="https://2010.igem.org/Team:METU_Turkey/Future_Work">Future Work</a> |
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<div align="center" style="margin:15px 0px 0px 0px"><img style="border: 0px solid ; width: 950px; height: 300px;" alt="w7" src="http://partsregistry.org/wiki/images/d/d0/Schedule.JPG"></a></div>
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<br><h2>PLANNING</h2>
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<br>
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<br>
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<br><h3>Cloning</h3>
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<br> - Design and ordering of DNA parts
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<br> - Preparation of competent cells
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<br> - Cloning
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<br> - Construction of Cell Sensor modules
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<br> - Validation
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<br> - Assembly of final construct
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<br>
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<br><h3>Cell Sensor Experiments</h3>
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<br> - Fermentor studies
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<br> - Flask experiments
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<br> - Solid culture experiments
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<br> - Myoglobin assay optimization
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<br> - Signal quantification with fluorescence spectroscopy
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<br> - Imaging with confocal laser scanning microscopy
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<br>
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<br><h3>CooA Expression and Purification</h3>
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<br> - Overexpression optimization
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<br> - Purification optimization
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<br>
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<br><h3>Characterization: Isothermal Titration Calorimetry (ITC)</h3>
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<br> - DNA component preparation
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<br> - Optimization studies
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<br> - Determination of Response Element - Transcription Factor Affinities
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<br>
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<br><h3>Characterization: Electrophoretic Mobility Shift Assay (EMSA)</h3>
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<br> - Optimization studies
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<br> - Determination of Response Element - Transcription Factor Affinities
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<br>
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<br><h3>Characterization: Intrinsic Tryptophan Fluorescence (ITF)</h3>
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<br> - Optimization studies
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<br> - Determination of Response Element - Transcription Factor Affinities
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<br>
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Latest revision as of 03:33, 28 October 2010

Home

 




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w7


PLANNING




Cloning


- Design and ordering of DNA parts
- Preparation of competent cells
- Cloning
- Construction of Cell Sensor modules
- Validation
- Assembly of final construct

Cell Sensor Experiments


- Fermentor studies
- Flask experiments
- Solid culture experiments
- Myoglobin assay optimization
- Signal quantification with fluorescence spectroscopy
- Imaging with confocal laser scanning microscopy

CooA Expression and Purification


- Overexpression optimization
- Purification optimization

Characterization: Isothermal Titration Calorimetry (ITC)


- DNA component preparation
- Optimization studies
- Determination of Response Element - Transcription Factor Affinities

Characterization: Electrophoretic Mobility Shift Assay (EMSA)


- Optimization studies
- Determination of Response Element - Transcription Factor Affinities

Characterization: Intrinsic Tryptophan Fluorescence (ITF)


- Optimization studies
- Determination of Response Element - Transcription Factor Affinities

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