Team:Newcastle/Meetings/4 June 2010

Informal Meeting - 4 June 2010

• Agenda

Urease tutorial - recap

• Accessory genes and nickel incorporation necessary more complicated than we thought.
• Software engineering cycle
• Requirements -> specification-> design-> implementation -> testing -> maintenance ...link back to the requirements
• Specification more detail on the requirements- literature
• Urease calcium carbonate, optimise for Bacillis
• Increase expression- what we thought!
• Biosynthesis of active BS in the Absence of Known Urease Accessory Proteins: paper
• Accessory genes required!
• Look over papers from tutorial
• Sporosarcina pasteurii
• Biobrick site symbols
• Promoter-ribosome- binding- coding sequence –ribosome binding site-coding-ribosome binding coding- terminator sequences ...and spacers
• pSpac
• ncbi – find the genome – can see the genes
• Implementation using sequencher
• Find the sequence of part
• Assemble parts
• Biobrick friendly restriction sites
• Genbank/embl file format
• Implementation altered by cloning strategy
• Biobricks RFC- request for comments
• [http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_10:_Draft_standard_for_Biobrick_biological_parts duplo and lego don’t fit together]
• [http://www.youtube.com/watch?v=FRijFX0BpGI&feature=player_embedded Video for lego pipette]

LAB TRAINING GROUPS:

• Group 1 starts 14th June: SW, D.Y AK, (HS); working on Biobrick 2 & 5 (End of crack & signalling system; Glue & lysis)

• Group 2 starts 21st June: JS, RB, RC, PH, (YE); working on BioBrick 3 & 4 (Non-target-environment kill switch; Spider silk and filamentous cells)

• All team members working on BioBrick 1 (CaCO3/Urease).
• RC working on BioBrick 6 (sequential programming)

• Team members not in the lab training will be working on modeling.
• HS and YE will unfortunately be away for both lab training sessions, hence the brackets