Team:Calgary/24 June 2010
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- | + | Thursday June 24, 2010| | |
- | + | [[Image:06.24.2010.E0040+B0015RestrictionDigest(2).jpg|thumb|400px|Dev's gel of restriction digest of E0040+B0015]] | |
<u>Emily</u> | <u>Emily</u> | ||
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Today, I restriction digested the construction of E0040+B0015 that was constructed on Monday. This would help identify the identity of the construct. I expected to see bands in the 3000 and 800 range however, bands were found in the | Today, I restriction digested the construction of E0040+B0015 that was constructed on Monday. This would help identify the identity of the construct. I expected to see bands in the 3000 and 800 range however, bands were found in the | ||
4000-3000 range which could be uncut plasmids | 4000-3000 range which could be uncut plasmids | ||
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+ | <u>Alex</u> | ||
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+ | The previous construction with the E0430 CFP device showed no fluorescence. Also grew overnight colonies of the cpxp construction using the colonies not expressing RFP. We did 10 colonies, labeling them C1-C10. | ||
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Latest revision as of 03:18, 23 August 2010
![University of Calgary iGEM Team 2010](http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Header4.png)
Thursday June 24, 2010
Emily
Today primers came in for the maltose binding protein. After diluting them, we set up a PCR with a variety of different colonies in order to try to PCR this gene about of the genome of E Coli.
Patrick
The previous day's R0040-E0430 and R0040-E0420 constructs grew colonies, but there was no fluorescence visible. I will leave this for another day just to see. As well, I selected five colonies from each plate of cpxP in pSB1A3. They have been put into an overnight culture for miniprepping tomorrow.
Dev
Today, I restriction digested the construction of E0040+B0015 that was constructed on Monday. This would help identify the identity of the construct. I expected to see bands in the 3000 and 800 range however, bands were found in the 4000-3000 range which could be uncut plasmids
Alex
The previous construction with the E0430 CFP device showed no fluorescence. Also grew overnight colonies of the cpxp construction using the colonies not expressing RFP. We did 10 colonies, labeling them C1-C10.