Team:Alberta/Notebook/protocols/overnight
From 2010.igem.org
(Difference between revisions)
(One intermediate revision not shown) | |||
Line 3: | Line 3: | ||
{{Team:Alberta/navbar|notebook=selected}} | {{Team:Alberta/navbar|notebook=selected}} | ||
- | {{Team:Alberta/beginLeftSideBar}} | + | {{Team:Alberta/beginLeftSideBar|toc=NO}} |
+ | |||
+ | |||
+ | ==General Protocols== | ||
+ | ----------------------------- | ||
+ | *[[Team:Alberta/Notebook/protocols/invitro_biobyte_assembly | In Vitro BioByte Assembly]] | ||
+ | ----------------------------- | ||
+ | *[[Team:Alberta/Notebook/protocols/LB | LB Plates and Broth]] | ||
+ | |||
+ | *[[Team:Alberta/Notebook/protocols/transformations |Transformations]] | ||
+ | |||
+ | *[[Team:Alberta/Notebook/protocols/overnight |5mL Overnight ]] | ||
+ | |||
+ | *[[Team:Alberta/Notebook/protocols/glycerol | Glycerol Stock ]] | ||
+ | ----------------------------- | ||
+ | *[[Team:Alberta/Notebook/protocols/miniprep | Plasmid Miniprep ]] | ||
+ | |||
+ | *[[Team:Alberta/Notebook/protocols/digest | Restriction Digest ]] | ||
+ | |||
+ | *[[Team:Alberta/Notebook/protocols/vector_dephos | Vector Dephosphorylation]] | ||
+ | |||
+ | *[[Team:Alberta/Notebook/protocols/ligation | Ligation ]] | ||
+ | ----------------------------- | ||
+ | *[[Team:Alberta/Notebook/protocols/agarose_gel | Agarose Gel Electrophoresis ]] | ||
+ | |||
+ | *[[Team:Alberta/Notebook/protocols/gel_extraction | Gel Extraction ]] | ||
+ | ----------------------------- | ||
+ | *[[Team:Alberta/Notebook/protocols/pcr | PCR]] | ||
+ | |||
+ | *[[Team:Alberta/Notebook/protocols/colony_pcr | Colony PCR ]] | ||
+ | |||
+ | *[[Team:Alberta/Notebook/protocols/pcr_purification | PCR Purification ]] | ||
+ | ----------------------------- | ||
+ | *[[Team:Alberta/Notebook/protocols/labelling | Sample Labelling Conventions]] | ||
+ | |||
+ | *[[Team:Alberta/Notebook/protocols/sequencing | Fluorescent Sequencing Reaction]] | ||
+ | |||
+ | *[[Team:Alberta/Notebook/protocols/primer_design | Primer Design]] | ||
+ | ----------------------------- | ||
+ | |||
+ | |||
+ | {{Team:Alberta/endMainContent}} | ||
+ | |||
+ | |||
{{Team:Alberta/endLeftSideBar}} | {{Team:Alberta/endLeftSideBar}} | ||
Line 11: | Line 54: | ||
{{Team:Alberta/beginMainContent}} | {{Team:Alberta/beginMainContent}} | ||
- | == | + | ==5mL Overnights== |
Line 35: | Line 78: | ||
- | |||
{{Team:Alberta/endMainContent}} | {{Team:Alberta/endMainContent}} |
Latest revision as of 02:21, 27 October 2010
5mL Overnights
Reagents:
- 10mL culture tube Use 16mm x 160mm or 16mm x 125 mm
- 5mL LB
- Concentrated antibiotics
- Single colonies on a plate (Best not to start O/N from glycerol stocks)
Procedure:
- Pipet 1.5-20uL of the appropriate antibiotic to the appropriate working concentration into culture tube.
- Add 5mL non-contaminated LB. Then add antibiotic.
- Select single colony using a sterile instrument that has been cooled and inoculate the liquid culture.
- Place culture tube in incubator at 37oC overnight shaking vigorously (250 rpm).
Notes:
For I0500, culture overnight; then add 1mM ITPG in morning and continue culture for another 3 hours. Then miniprep.