Team:Alberta/Tour/biobytes

From 2010.igem.org

(Difference between revisions)

Latest revision as of 02:36, 28 October 2010

TEAM ALBERTA

Start BioBytes 2.0 The Kit The Software Achievements Conclusion

Accelerated Construction

This year's project required a new approach to assembling DNA constructs. The need for a fast and efficient assembly standard was filled by BioBytes 2.0. BioBytes 2.0 is an innovative step forward from last year's BioBytes 1.0.

The new assembly method utilizes iron micro beads to anchor the DNA at a specified end. This allows for the DNA Bytes to be added in a controlled and sequential manner, ensuring the proper orientation and order of Bytes. This is made possible because of the design of our unique anchor part, which attaches to the iron micro bead at one end and to subsequent Bytes on the other. With the addition of our unique terminal cap Byte, the assembled construct will be able to circularize, and can then be transformed into E. coli.

@@ Line 5: / Line 5: @@
 {{Team:Alberta/beginLeftSideBar|toc=NO|class=not-top}}
+[[Image:Team-alberta-biobyteprocess-tour.jpg|right|280px|thumb|This is the BioByte 2.0 system]]
 {{Team:Alberta/endLeftSideBar}}
 {{Team:Alberta/beginRightSideBar|class=not-top}}
+[[Image:Alberta_BioByte2.0vs1.0.png|left|280px|thumb|GENOMIKON's BioByte 2.0 vs BioByte 1.0]]
+<p></p>
 {{Team:Alberta/endRightSideBar}}
 {{Team:Alberta/beginMainContent|class=not-top}}
-We continued to develop the biobytes assembly method developed by last year’s team.
-The method has three main components:
-==The Anchor==
-A ferro-magnetic bead attached to a piece of DNA. This piece serves as the initial piece from which we assemble a DNA construct.  The bead allows us to manipulate the DNA with magnets making washing and subsequent attachments easier.
-[[Image:team-alberta-anchor-tour.jpg|center]]
-==The bytes==
-DNA fragments that can be attached together to build up a larger construct. There are two types  of pieces, AB and BA.  The A end can join only with another A end and the B end can join only with another B end.  As a result pieces can only be joined in a single orientation.
-[[Image:team-alberta-bytes-tour.jpg|center]]
-==The cap==
-A DNA fragment that finishes off a construct and allows for circularization of the construct into a plasmid.
-[[Image:team-alberta-cap-tour.jpg|center]]
-==Putting the pieces together==
-The process of building a plasmid is more elegant and more rapid than the current biobyte system!
-[[Image:team-alberta-biobyteprocess-tour.jpg]]
-Easy! And fast too, each step takes about 7 minutes.
-[[Image:team-alberta-building-tour.jpg|center]]
-Compare that to the standard biobrick method:
-[[Image:team-alberta-biobrick-tour.jpg|center]]
-Using this process we were able to assemble eight pieces in an afternoon!
+==Accelerated Construction==
+<div id="horiz-line"></div>
+<p>
+This year's project required a new approach to assembling DNA constructs. The need for a fast and efficient assembly standard was filled by BioBytes 2.0. BioBytes 2.0 is an innovative step forward from last year's BioBytes 1.0.
+</p><p>
+The new assembly method utilizes iron micro beads to anchor the DNA at a specified end. This allows for the DNA Bytes to be added in a controlled and sequential manner, ensuring the proper orientation and order of Bytes. This is made possible because of the design of our unique anchor part, which attaches to the iron micro bead at one end and to subsequent Bytes on the other. With the addition of our unique terminal cap Byte, the assembled construct will be able to circularize, and can then be transformed into ''E. coli''.
+</p>
 {{Team:Alberta/endMainContent}}
-{{Team:Alberta/tourbar|biobytes=selected|left=start|right=the_kit|class=no-top}}