Team:Alberta/Tour/biobytes

From 2010.igem.org

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[[Image:Team-alberta-biobyteprocess-tour.jpg|right|280px|thumb|This is the BioByte 2.0 system]]
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[[Image:Alberta_BioByte2.0vs1.0.png|left|280px|thumb|GENOMIKON's BioByte 2.0 vs BioByte 1.0]]
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We continued to develop the biobytes assembly method developed by last year’s team.
 
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The method has three main components:
 
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[[Image:team-alberta-anchor-tour.jpg]]
 
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The Anchor: a ferro-magnetic bead attached to a piece of DNA. This piece serves as the initial piece from which we assemble a DNA construct.  The bead allows us to manipulate the DNA with magnets making washing and subsequent attachments easier.
 
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[[Image:team-alberta-bytes-tour.jpg]]
 
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The bytes: DNA fragments that can be attached together to build up a larger construct. There are two types  of pieces, AB and BA.  The A end can join only with another A end and the B end can join only with another B end.  As a result pieces can only be joined in a single orientation.
 
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[[Image:team-alberta-cap-tour.jpg]]
 
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The cap: a DNA fragment that finishes off a construct and allows for circularization of the construct into a plasmid.
 
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[[Image:team-alberta-biobyteprocess-tour.jpg]]
 
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The process of building a plasmid is elegant and rapid! 
 
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[[Image:team-alberta-building-tour.jpg]]
 
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[[Image:team-alberta-biobrick-tour.jpg]]
 
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Starting with an anchor, add the first piece and ligate.
 
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Then hold the single piece construct in the tube by placing it on the magnetic rack.  Now you can wash away most of the excess of piece 1.
 
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Add the next piece and repeat until you have added all the pieces you want.
 
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Then add the cap.
 
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Now just heat to release the anchor and open up the cap, upon cooling the construct will circularize. 
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==Accelerated Construction==
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Easy!
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This year's project required a new approach to assembling DNA constructs. The need for a fast and efficient assembly standard was filled by BioBytes 2.0. BioBytes 2.0 is an innovative step forward from last year's BioBytes 1.0.
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The new assembly method utilizes iron micro beads to anchor the DNA at a specified end. This allows for the DNA Bytes to be added in a controlled and sequential manner, ensuring the proper orientation and order of Bytes. This is made possible because of the design of our unique anchor part, which attaches to the iron micro bead at one end and to subsequent Bytes on the other. With the addition of our unique terminal cap Byte, the assembled construct will be able to circularize, and can then be transformed into ''E. coli''.
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Using this process we were able to assemble eight pieces in an afternoon!
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Latest revision as of 02:36, 28 October 2010

TEAM ALBERTA

Accelerated Construction

This year's project required a new approach to assembling DNA constructs. The need for a fast and efficient assembly standard was filled by BioBytes 2.0. BioBytes 2.0 is an innovative step forward from last year's BioBytes 1.0.

The new assembly method utilizes iron micro beads to anchor the DNA at a specified end. This allows for the DNA Bytes to be added in a controlled and sequential manner, ensuring the proper orientation and order of Bytes. This is made possible because of the design of our unique anchor part, which attaches to the iron micro bead at one end and to subsequent Bytes on the other. With the addition of our unique terminal cap Byte, the assembled construct will be able to circularize, and can then be transformed into E. coli.

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