Team:Newcastle/Meetings/21 October 2010

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*Apologies - Phil, Younus, Deena late
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==Formal Meeting - 21st October 2010==
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*Action point: Phil and Rachel add characteristation data to the wiki
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==''yneA'' Characteristaion:==
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*Apologies - Phil, Younus, Deena (late)
 +
*[[Team:Newcastle/Agendas/21_October_2010|Agenda]]
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*Δ''amyE'':Pspac(hy)::''yneA''
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====''yneA'' Characteristaion:====
 +
 
 +
*Δ''amyE'':Pspac(hy)-oid::''yneA''
*''B. subtilis'' spread of IPTG may not be homogenous through the population.  
*''B. subtilis'' spread of IPTG may not be homogenous through the population.  
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*Younus AP: send script for yneA to Jen, Matt, Neil  
*Younus AP: send script for yneA to Jen, Matt, Neil  
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*Plot: Max cell length against ITPG + Error bars  
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*Plot: Max cell length against ITPG + Error bars
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==Arabinose==  
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====Arabinose====  
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We think we have it cloned infront of GFP  
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* We think we have it cloned infront of GFP  
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''Bacillus subtilis'' transformation tommorrow  
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*''B. subtilis'' transformation tommorrow  
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Fluorescence  
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*Fluorescence  
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'''Characterisation of previous part!!!'''
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*Characterisation of previous part!!!
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==Concrete==  
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====Concrete====
* Damaged building - earthquake  
* Damaged building - earthquake  
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==Flux balance==  
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====Flux balance====  
*difference between optimal and normal
*difference between optimal and normal
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==Lab work==  
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====Lab work====  
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DNA submitted... tracking number sent
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1C3 ''SR1'' '''has not arrived''', we would have had 2 bricks submitted!
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-
 
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==Safety page==  
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-
 
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Deena and Harsh
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Risk assessment- ''Bacillus''
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BioCOSH
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*DNA submitted - tracking number sent
 +
*1C3 ''SR1'' '''has not arrived''', we would have had 2 bricks submitted!
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Safety Guy talk mention it
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====Safety page====
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==Presentation==
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*Deena and Harsh
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'''Phil''' DON'T BE AGGRESSIVE!!!!
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*Risk assessment - ''Bacillus''  
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Beginning:
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*BioCOSH
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*say what the project is about
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*mention ''Bacillus subtilis''
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*Why bacillus?
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*bioconcrete- show its current
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*Non-pathogenic not non-virulent
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*Previous work on CaCO<sub>3</sub>
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*Don't use the word '''PROVES'''
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*Separate slid for work flows
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*Alan - Flux first, speak slowly
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*Summary table- redo
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*BRONZE, SILVER, GOLD!!!!!!! (see below)
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===GOLD===
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*Safety Guy talk mention it
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Jannetta write RFC
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*number on judging form
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*webservice!!!
+
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===SEM===  
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====Presentation====
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Images are ours
+
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==Other==
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*Phil - DON'T BE AGGRESSIVE!!!!
 +
*Beginning:
 +
**say what the project is about
 +
**mention ''Bacillus subtilis''
 +
**Why bacillus?
 +
**bioconcrete- show its current
 +
**Non-pathogenic not non-virulent
 +
*BioBricks:
 +
**Previous work on CaCO<sub>3</sub>
 +
**Don't use the word '''PROVES'''
 +
**Alan - Flux first, speak slowly
 +
*Gold:
 +
**Jannetta write RFC
 +
**number on judging form
 +
**webservice!!!
 +
**Separate slid for work flows
 +
*SEM:
 +
**Images are ours
 +
*Summary table
 +
**redo with BRONZE, SILVER, GOLD section?!
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Insurance form
+
====Action points:====
-
Taxi
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*Check presentation and poster submission. Poster next Thursday due in? 
 +
*Phil and Rachel to add characteristation data to the wiki
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Younus - Poster next thursday due in? 
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====Other====
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Action point check presentation and poster submission!!!
+
*Insurance form
 +
*Taxi
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Latest revision as of 10:44, 27 October 2010

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Contents

Formal Meeting - 21st October 2010

  • Apologies - Phil, Younus, Deena (late)
  • Agenda

yneA Characteristaion:

  • ΔamyE:Pspac(hy)-oid::yneA
  • B. subtilis spread of IPTG may not be homogenous through the population.
  • E. coli has transporter deltions: check E. coli wiki
  • take out pMap65 results
  • Median, Mode add to graph
  • pMutin4 proportion of cells
  • 20% responding- looks like
  • Biomass of cells - length * amount (counts)) against IPTG
  • Lysis acts to counter the overexpression of yneA ... so we have fewer cells
  • Younus AP: send script for yneA to Jen, Matt, Neil
  • Plot: Max cell length against ITPG + Error bars

Arabinose

  • We think we have it cloned infront of GFP
  • B. subtilis transformation tommorrow
  • Fluorescence
  • Characterisation of previous part!!!

Concrete

  • Damaged building - earthquake

Flux balance

  • difference between optimal and normal

Lab work

  • DNA submitted - tracking number sent
  • 1C3 SR1 has not arrived, we would have had 2 bricks submitted!

Safety page

  • Deena and Harsh
  • Risk assessment - Bacillus
  • BioCOSH
  • Safety Guy talk mention it

Presentation

  • Phil - DON'T BE AGGRESSIVE!!!!
  • Beginning:
    • say what the project is about
    • mention Bacillus subtilis
    • Why bacillus?
    • bioconcrete- show its current
    • Non-pathogenic not non-virulent
  • BioBricks:
    • Previous work on CaCO3
    • Don't use the word PROVES
    • Alan - Flux first, speak slowly
  • Gold:
    • Jannetta write RFC
    • number on judging form
    • webservice!!!
    • Separate slid for work flows
  • SEM:
    • Images are ours
  • Summary table
    • redo with BRONZE, SILVER, GOLD section?!

Action points:

  • Check presentation and poster submission. Poster next Thursday due in?
  • Phil and Rachel to add characteristation data to the wiki

Other

  • Insurance form
  • Taxi
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