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Team:Newcastle/Meetings/21 October 2010
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| - | + | ==Formal Meeting - 21st October 2010== | |
| - | + | *Apologies - Phil, Younus, Deena (late) | |
| + | *[[Team:Newcastle/Agendas/21_October_2010|Agenda]] | ||
| - | ==''yneA'' Characteristaion:== | + | ====''yneA'' Characteristaion:==== |
| - | *Δ''amyE'':Pspac(hy)::''yneA'' | + | *Δ''amyE'':Pspac(hy)-oid::''yneA'' |
| - | *'' | + | *''B. subtilis'' spread of IPTG may not be homogenous through the population. |
| - | *''E.coli'' has transporter deltions: check ''E.coli'' wiki | + | *''E. coli'' has transporter deltions: check ''E. coli'' wiki |
| - | take out pMap65 results | + | *take out pMap65 results |
| - | Median, Mode add to graph | + | *Median, Mode add to graph |
| - | pMutin4 proportion of cells | + | *pMutin4 proportion of cells |
| - | 20% responding- looks like | + | *20% responding- looks like |
| - | Biomass of cells - length * amount (counts)) against IPTG | + | *Biomass of cells - length * amount (counts)) against IPTG |
| - | Lysis acts to counter the overexpression of yneA ... so we have fewer cells | + | *Lysis acts to counter the overexpression of yneA ... so we have fewer cells |
| - | Younus AP: send script for yneA to Jen, Matt, Neil | + | *Younus AP: send script for yneA to Jen, Matt, Neil |
| - | Plot: Max cell length against ITPG + Error bars | + | *Plot: Max cell length against ITPG + Error bars |
| - | ==Arabinose== | + | ====Arabinose==== |
| - | We think we have it cloned infront of GFP | + | * We think we have it cloned infront of GFP |
| - | '' | + | *''B. subtilis'' transformation tommorrow |
| - | Fluorescence | + | *Fluorescence |
| - | + | *Characterisation of previous part!!! | |
| - | ==Concrete== | + | ====Concrete==== |
| - | Damaged building- earthquake | + | * Damaged building - earthquake |
| - | ==Flux balance== | + | ====Flux balance==== |
| - | difference between optimal and normal | + | *difference between optimal and normal |
| - | ==Lab work== | + | ====Lab work==== |
| - | DNA submitted | + | *DNA submitted - tracking number sent |
| - | 1C3 ''SR1'' '''has not arrived''', we would have had 2 bricks submitted! | + | *1C3 ''SR1'' '''has not arrived''', we would have had 2 bricks submitted! |
| - | ==Safety page== | + | ====Safety page==== |
| - | Deena and Harsh | + | *Deena and Harsh |
| - | Risk assessment- ''Bacillus'' | + | *Risk assessment - ''Bacillus'' |
| - | BioCOSH | + | *BioCOSH |
| - | Safety Guy talk mention it | + | *Safety Guy talk mention it |
| - | ==Presentation== | + | ====Presentation==== |
| - | ''' | + | *Phil - DON'T BE AGGRESSIVE!!!! |
| + | *Beginning: | ||
| + | **say what the project is about | ||
| + | **mention ''Bacillus subtilis'' | ||
| + | **Why bacillus? | ||
| + | **bioconcrete- show its current | ||
| + | **Non-pathogenic not non-virulent | ||
| + | *BioBricks: | ||
| + | **Previous work on CaCO<sub>3</sub> | ||
| + | **Don't use the word '''PROVES''' | ||
| + | **Alan - Flux first, speak slowly | ||
| + | *Gold: | ||
| + | **Jannetta write RFC | ||
| + | **number on judging form | ||
| + | **webservice!!! | ||
| + | **Separate slid for work flows | ||
| + | *SEM: | ||
| + | **Images are ours | ||
| + | *Summary table | ||
| + | **redo with BRONZE, SILVER, GOLD section?! | ||
| - | + | ====Action points:==== | |
| - | * | + | *Check presentation and poster submission. Poster next Thursday due in? |
| - | + | *Phil and Rachel to add characteristation data to the wiki | |
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| - | + | ====Other==== | |
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| - | + | *Insurance form | |
| - | + | *Taxi | |
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{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Latest revision as of 10:44, 27 October 2010
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Contents |
Formal Meeting - 21st October 2010
- Apologies - Phil, Younus, Deena (late)
- Agenda
yneA Characteristaion:
- ΔamyE:Pspac(hy)-oid::yneA
- B. subtilis spread of IPTG may not be homogenous through the population.
- E. coli has transporter deltions: check E. coli wiki
- take out pMap65 results
- Median, Mode add to graph
- pMutin4 proportion of cells
- 20% responding- looks like
- Biomass of cells - length * amount (counts)) against IPTG
- Lysis acts to counter the overexpression of yneA ... so we have fewer cells
- Younus AP: send script for yneA to Jen, Matt, Neil
- Plot: Max cell length against ITPG + Error bars
Arabinose
- We think we have it cloned infront of GFP
- B. subtilis transformation tommorrow
- Fluorescence
- Characterisation of previous part!!!
Concrete
- Damaged building - earthquake
Flux balance
- difference between optimal and normal
Lab work
- DNA submitted - tracking number sent
- 1C3 SR1 has not arrived, we would have had 2 bricks submitted!
Safety page
- Deena and Harsh
- Risk assessment - Bacillus
- BioCOSH
- Safety Guy talk mention it
Presentation
- Phil - DON'T BE AGGRESSIVE!!!!
- Beginning:
- say what the project is about
- mention Bacillus subtilis
- Why bacillus?
- bioconcrete- show its current
- Non-pathogenic not non-virulent
- BioBricks:
- Previous work on CaCO3
- Don't use the word PROVES
- Alan - Flux first, speak slowly
- Gold:
- Jannetta write RFC
- number on judging form
- webservice!!!
- Separate slid for work flows
- SEM:
- Images are ours
- Summary table
- redo with BRONZE, SILVER, GOLD section?!
Action points:
- Check presentation and poster submission. Poster next Thursday due in?
- Phil and Rachel to add characteristation data to the wiki
Other
- Insurance form
- Taxi
   
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