Team:KIT-Kyoto/Parts

From 2010.igem.org

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{{Template:KIT-Kyoto/menu}}
{{Template:KIT-Kyoto/menu}}
<table border=0 width="965px" align="center"><tr><td>
<table border=0 width="965px" align="center"><tr><td>
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<div aling="left">[[Team:KIT-Kyoto|Home]] > [[Team:KIT-Kyoto/Parts|Parts]]</div></td><td><div align="right">Language : [[Team:KIT-Kyoto/Parts|English]] / [[Team:KIT-Kyoto/PartsJ|Japanese]]</div></td></tr></table>
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<div aling="left">[[Team:KIT-Kyoto/Home|Home]] > [[Team:KIT-Kyoto/Parts|Parts]]</div></td><td><div align="right">Language : [[Team:KIT-Kyoto/Parts|English]] / [[Team:KIT-Kyoto/PartsJ|Japanese]]</div></td></tr></table>
<div id="NAKAMI">
<div id="NAKAMI">
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== We use these parts for making original biobrick parts ==
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== We used these parts for making our original biobrick parts ==
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=== 1.We get these parts from 2010 iGEM kit ===
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=== 1.We obtained these parts from 2010 iGEM kit ===
{| style="margin: 1em auto 1em auto" width="750px"
{| style="margin: 1em auto 1em auto" width="750px"
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|<partinfo>pSB1AK3</partinfo>(<partinfo>BBa_I732950</partinfo>)||Protein domain||Kanamycin||3230bp||2079bp||RBS+LacZ+T+T
|<partinfo>pSB1AK3</partinfo>(<partinfo>BBa_I732950</partinfo>)||Protein domain||Kanamycin||3230bp||2079bp||RBS+LacZ+T+T
|- style="background-color:#eaf4fc;"
|- style="background-color:#eaf4fc;"
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|<partinfo>pSB4A5</partinfo>(<partinfo>BBa_K193602</partinfo>)||Composite||Ampicillin||1896bp||3395bp||promoter(Lac)+RBS+melA+T+T|
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|<partinfo>pSB4A5</partinfo>(<partinfo>BBa_K193602</partinfo>)||Composite||Ampicillin||1896bp||3395bp||promoter(LacIQ)+RBS+melA
|- style="background-color:#eaf4fc;"
|- style="background-color:#eaf4fc;"
|<partinfo>pSB1A2</partinfo>(<partinfo>BBa_E0240</partinfo>)||Protein domain||Ampicillin||883bp||2079bp||RBS+GFP+T+T
|<partinfo>pSB1A2</partinfo>(<partinfo>BBa_E0240</partinfo>)||Protein domain||Ampicillin||883bp||2079bp||RBS+GFP+T+T
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=== 2.We get this part directly from iGEM HQ ===
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=== 2.We obtained this part directly from iGEM HQ ===
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We characterize this BioBrick Part and enter the new information back on the Registry. This parts don’t include detailed information on pSB6 and the reasons leading to its usage in our work to produce GFP. In general, pSB6 is a low copy plasmid which makes it more suitable for protein production than the high copy vector. However this information cannot be found in any sites related to iGEM. Consequently, we tried to compare the performances of the low copy vector pSB1 and pSB6. We have confirmed in this way that pSB6 is more efficient at producing GFP protein than PSB1. We have thus proved that this part is very useful to evaluate the capabilities of a promoter, in this case by observing the degree of fluorescence of GFP protein. We are very thankful to the 08’Tokyo-Tech group who has supplied us with this part.
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:We added new information to the BioBrick Part (pSB6A1) shown below that was originally developed by the 09’Tokyo-Tech group. This BioBrick Part having the backbone of pSB6, a low copy plasmid was designed to express GFP in <I>E. coli</I>. Generally low copy plasmid is more efficient in protein expression in compared with high copy plasmid such as pSB1. However no quantitative data on the efficiency of GFP expression with this plasmid was described in any site of iGEM. We therefore measured intensities of fluorescence of GFP protein produced in <I>E. coli</I> carrying the pSB6A1 and compared with that carrying the pSB1A2. The data clearly showed that expression level of GFP is much higher in E. coli carrying pSB6A1 than that carrying pSB1A2. Thus we decided to use pSB6 vector to measure activities of various promoters responsible to H<sub>2</sub>O<sub>2</sub>. We thank the 09’Tokyo-Tech group who has originally developed this part.This new information would be useful for the other iGEM teams in future.
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<BR>
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:[https://2010.igem.org/Team:KIT-Kyoto/Project/Abstract#2._Compare_the_performances_of_the_high_copy_vector_and_low_copy_vector >>About pSB1 vs pSB6]
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== We make these parts originally. ==
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== We designed and constructed these parts originally. ==
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We cloned the following inserts into the vector, pSB6A1. Out of them we cloned the two inserts(BBa_K362005, BBa_K362007) into the pSB1C3 to send to the iGEM headquarter.
<div align="center">
<div align="center">
<groupparts>iGEM010 KIT-Kyoto</groupparts>
<groupparts>iGEM010 KIT-Kyoto</groupparts>
</div>
</div>
{{Template:KIT-Kyoto-1}}
{{Template:KIT-Kyoto-1}}

Latest revision as of 03:16, 27 October 2010



Home > Parts
Language : English / Japanese

We used these parts for making our original biobrick parts

1.We obtained these parts from 2010 iGEM kit

NamePart typeResistanceInsertVectorContents
<partinfo>pSB3k3</partinfo>(<partinfo>BBa_J04450</partinfo>)Plasmid backboneKanamycin738bp2750bppromoter(LacI) +RBS+mRFP+T+T
<partinfo>pSB6A1</partinfo>(<partinfo>BBa_J04450</partinfo>)Plasmid backboneAmpicillin738bp4032bppromoter(LacI) +RBS+mRFP+T+T
<partinfo>pSB1C3</partinfo>(<partinfo>BBa_J04450</partinfo>)Plasmid backboneChloramphenicol1069bp2072bppromoter(LacI) +RBS+mRFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_I3522</partinfo>)CompositeAmpicillin937bp2079bppromoter(TetR)+RBS+GFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_R0040</partinfo>)RegulatoryAmpicillin54bp2079bppromoter(TetR)
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_I732019</partinfo>)RegulatoryAmpicillin3230bp2079bpRBS+LacZ+T+T
<partinfo>pSB1AK3</partinfo>(<partinfo>BBa_I732950</partinfo>)Protein domainKanamycin3230bp2079bpRBS+LacZ+T+T
<partinfo>pSB4A5</partinfo>(<partinfo>BBa_K193602</partinfo>)CompositeAmpicillin1896bp3395bppromoter(LacIQ)+RBS+melA
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_E0240</partinfo>)Protein domainAmpicillin883bp2079bpRBS+GFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_I13507</partinfo>)Protein domainAmpicillin937bp2079bpRBS+RFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_E0420</partinfo>)Protein domainAmpicillin878bp2079bpRBS+CFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_E0430</partinfo>)Protein domainAmpicillin878bp2079bpRBS+YFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_I13600</partinfo>)CompositeAmpicillin940bp2079bppromoter(TetR)+RBS+CFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_J22005</partinfo>)CompositeAmpicillin2079bp2623bppromoter(TetR)+RBS+YFP+T+T

2.We obtained this part directly from iGEM HQ

We added new information to the BioBrick Part (pSB6A1) shown below that was originally developed by the 09’Tokyo-Tech group. This BioBrick Part having the backbone of pSB6, a low copy plasmid was designed to express GFP in E. coli. Generally low copy plasmid is more efficient in protein expression in compared with high copy plasmid such as pSB1. However no quantitative data on the efficiency of GFP expression with this plasmid was described in any site of iGEM. We therefore measured intensities of fluorescence of GFP protein produced in E. coli carrying the pSB6A1 and compared with that carrying the pSB1A2. The data clearly showed that expression level of GFP is much higher in E. coli carrying pSB6A1 than that carrying pSB1A2. Thus we decided to use pSB6 vector to measure activities of various promoters responsible to H2O2. We thank the 09’Tokyo-Tech group who has originally developed this part.This new information would be useful for the other iGEM teams in future.


>>About pSB1 vs pSB6
NamePart TypeResistanceInsertVector
<partinfo>pSB6A1</partinfo>(<partinfo>BBa_K121013</partinfo>)Protein domainAmpicillin883bp4022bpRBS+GFP+T+T

We designed and constructed these parts originally.

We cloned the following inserts into the vector, pSB6A1. Out of them we cloned the two inserts(BBa_K362005, BBa_K362007) into the pSB1C3 to send to the iGEM headquarter.

<groupparts>iGEM010 KIT-Kyoto</groupparts>


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