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Team:Newcastle/1 July 2010
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*To make a stock of competent ''E. coli'' (DH5α strain) ready for transformation. | *To make a stock of competent ''E. coli'' (DH5α strain) ready for transformation. | ||
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* Set up [[Team:Newcastle/Growing a liquid culture| liquid culture]] consisting of ''E. coli'' DH5α. | * Set up [[Team:Newcastle/Growing a liquid culture| liquid culture]] consisting of ''E. coli'' DH5α. | ||
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{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Latest revision as of 14:11, 25 October 2010
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Contents |
Urease Test
Aims
The aim of this experiment was to determine if Bacillus subtilis 168 is able to produce urease and degrade urea.
Procedures
- Please refer to urease test
Inference
Christensen's Urea Agar was formulated to detect and differentiate urolytic and urea degrading microorganisms. The addition of gelatine peptone, dextrose and reduced content of buffer supports a luxuriant growth at an early stage. Urea is the substrate and can be degraded by certain organisms, which results in ammonia building. The ammonia makes the media alkaline and therefore the indicator phenol red will change from orange color to pink-red color.
- Negative control - No color change (Orange color)
- Test 1 (Duplicate) - Pink-red color
- Test 2 (Duplicate) - Pink-red color
For actual results, please see 02.07.10 lab notebook.
Competent E. coli Production
Aims
- To make a stock of competent E. coli (DH5α strain) ready for transformation.
Materials and Protocol
- Set up liquid culture consisting of E. coli DH5α.
   
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