Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/10

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Header}}
=2010/10/10=
=2010/10/10=
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===1:Electrophoresis===
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<Member><br />
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nito
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<Sample><br />
 +
PCR products (10/10)
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 +
<Protocol><br />
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
<Result><br />
<Result><br />
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<Result><br />
<Result><br />
[[Image: Image:Matsuura 2010-10-10 21hr 24min.JPG]]
[[Image: Image:Matsuura 2010-10-10 21hr 24min.JPG]]
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 +
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===2:PCR===
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<Member><br />
 +
nito
 +
 +
<Sample>(temperature in annealing:℃)<br />
 +
*9 (60)
 +
*1-2 (60)
 +
*2-10 (60)
 +
*7-8-10 (60)
 +
 +
<Protocol><br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
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 +
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===3：DNA Digestion===
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＜Member＞<br />
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nito
 +
 +
＜Sample, Materials＞<br />
 +
・PCR productions
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*5
 +
 +
・Digest enzyme
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*Spe1
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 +
＜Protocol＞<br />
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
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 +
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===4：DNA Ligation ===
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＜Member＞<br />
 +
nito
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 +
＜Sample＞<br />
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*1-2 
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*10-2
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(after digestion)
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 +
＜Protocol＞<br />
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
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 +
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===5：DNA extraction===
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＜Member＞<br />
 +
Mariko,mio,nito
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 +
＜Sample＞<br />
 +
PCR products
 +
*2
 +
*4
 +
*8
 +
*10
 +
*5
 +
 +
＜Protocol＞<br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
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 +
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===6:Plasmid extraction from E-coli===
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<Member><br />
 +
nito
 +
 +
<Sample><br />
 +
*12
 +
*13
 +
*15
 +
*16
 +
 +
<Protocol><br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Plasmid_extraction_from_E-coli/ Protocol 6 ]

Revision as of 13:56, 12 October 2010


Contents

2010/10/10

1:Electrophoresis

<Member>
nito

<Sample>
PCR products (10/10)

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-10 11hr 40min.JPG

<Result>
Matsuura 2010-10-10 21hr 24min.JPG

<Result>
Matsuura 2010-10-10 18hr 40min.JPG

<Result>
File:Image:Matsuura 2010-10-10 21hr 24min.JPG


2:PCR

<Member>
nito

<Sample>(temperature in annealing:℃)

  • 9 (60)
  • 1-2 (60)
  • 2-10 (60)
  • 7-8-10 (60)

<Protocol>
See Protocol 1


3：DNA Digestion

＜Member＞
nito

＜Sample, Materials＞
・PCR productions

  • 5

・Digest enzyme

  • Spe1

＜Protocol＞
See Prptpcpl 9


4：DNA Ligation

＜Member＞
nito

＜Sample＞

  • 1-2
  • 10-2

(after digestion)

＜Protocol＞
See Protocol 10


5：DNA extraction

＜Member＞
Mariko,mio,nito

＜Sample＞
PCR products

  • 2
  • 4
  • 8
  • 10
  • 5

＜Protocol＞
See Protocol 4


6:Plasmid extraction from E-coli

<Member>
nito

<Sample>

  • 12
  • 13
  • 15
  • 16

<Protocol>
See Protocol 6

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/10/10"