Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/09

From 2010.igem.org

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===4:Electrophoresis③===
===4:Electrophoresis③===

Revision as of 12:57, 12 October 2010


Contents

2010/10/09

1:PCR

<Member>
Hitomi

<Sample>(temperature in annealing:℃)

  • 1
  • 2
  • 3
  • 4
  • 5
  • 6
  • 7
  • 8
  • 9
  • 10
  • 1-2
  • 2-3
  • 2-7
  • 2-10
  • 4-5
  • 6-3
  • 6-7
  • 7-8-10

<Protocol>
See Protocol 1


2:Electrophoresis①

<Member>
Hitomi

<Sample>
PCR products

  • 10-2

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-09 14hr 11min.JPG

3:Electrophoresis②

<Member>
Hitomi

<Sample>
PCR products (10/8)

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-09 18hr 03min.JPG

4:Electrophoresis③

<Member>
Hitomi

<Sample>
PCR products (10/9)

<Protocol>
See Protocol 8

<Result>
[[Image:]]


5:Electrophoresis④

<Member>
Mariko

<Sample>
PCR products

  • 1
  • 2
  • 3
  • 4
  • 5
  • 6
  • 7
  • 8
  • 9
  • 10
  • 2-10
  • 4-5

<Protocol>
See Protocol 8

<Result>
[[Image:]]


6:DNA Digestion

<Member>
Mariko

<Sample, Materials>
・PCR productions

  • 1
  • 2
  • 3
  • 4
  • 6
  • 10
  • 7-8-10
  • vector

・Digest enzyme

  • Avr2
  • Nhe1
  • Spe1

<Protocol>
See Prptpcpl 9