Team:Lethbridge/Notebook/Lab Work/October
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=<font color="white">October= | =<font color="white">October= | ||
==<font color="white">October 2, 2010== | ==<font color="white">October 2, 2010== | ||
- | ===ADS=== | + | ===<font color="white">ADS=== |
<b>Objective:</b> Purify plasmid DNA from transformed Mr. GENE synthesized DNA | <b>Objective:</b> Purify plasmid DNA from transformed Mr. GENE synthesized DNA | ||
*<partinfo>K331022</partinfo> | *<partinfo>K331022</partinfo> | ||
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<b>Method:</b> Use Qiagen miniprep method with BioBasic EZ-10 Spin columns.<br> | <b>Method:</b> Use Qiagen miniprep method with BioBasic EZ-10 Spin columns.<br> | ||
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- | <b>Objective:</b> Create 20 new biobricks using recently arrived synthesized DNA | + | <b>Objective:</b> Create 20 new biobricks using recently arrived synthesized DNA and terminator <partinfo>B0015</partinfo>, pLac <partinfo>R0010</partinfo>, and pTet <partinfo>R0040</partinfo> to create the following new biobricks: |
+ | *1)R0010 + K331022 to obtain (<font color="red">NAME BIOBRICK!!!!</font>) | ||
+ | *2)R0040 + K331022 to obtain (<font color="red">NAME BIOBRICK!!!!</font>) | ||
+ | *3)K331022 + B0015 to obtain (<font color="red">NAME BIOBRICK!!!!</font>) | ||
+ | *4)K331022 + K331024 to obtain (<font color="red">NAME BIOBRICK!!!!</font>) | ||
+ | *5)R0010 + K331023 to obtain (<font color="red">NAME BIOBRICK!!!!</font>) | ||
+ | *6)R0040 + K331023 to obtain (<font color="red">NAME BIOBRICK!!!!</font>) | ||
+ | *7)K331023 + B0015 to obtain (<font color="red">NAME BIOBRICK!!!!</font>) | ||
+ | *8)K331023 + K331025 to obtain (<font color="red">NAME BIOBRICK!!!!</font>) | ||
+ | *9)R0010 + K331024 to obtain (<font color="red">NAME BIOBRICK!!!!</font>) | ||
+ | *10)R0040 + K331024 to obtain (<font color="red">NAME BIOBRICK!!!!</font>) | ||
+ | *11)K331024 + B0015 to obtain (<font color="red">NAME BIOBRICK!!!!</font>) | ||
+ | *12)K331024 + K331022 to obtain (<font color="red">NAME BIOBRICK!!!!</font>) | ||
+ | *13)R0010 + K331025 to obtain (<font color="red">NAME BIOBRICK!!!!</font>) | ||
+ | *14)R0040 + K331025 to obtain (<font color="red">NAME BIOBRICK!!!!</font>) | ||
+ | *15)K331025 + B0015 to obtain (<font color="red">NAME BIOBRICK!!!!</font>) | ||
+ | *16)K331025 + K331023 to obtain (<font color="red">NAME BIOBRICK!!!!</font>) | ||
+ | *17)K331022 to obtain (<font color="red">NAME BIOBRICK!!!!</font>) | ||
+ | *18)K331023 to obtain (<font color="red">NAME BIOBRICK!!!!</font>) | ||
+ | *19)K331024 to obtain (<font color="red">NAME BIOBRICK!!!!</font>) | ||
+ | *20)K331025 to obtain (<font color="red">NAME BIOBRICK!!!!</font>) | ||
+ | All parts will be inserted into pSB1C3. | ||
+ | <b>Method:</b> Use BioBrick Assembly Method<br> | ||
+ | <b>Results:</b> Obtained <font color="red">RESULTS!!!</font> positive colonies. | ||
+ | |||
+ | |||
<br> | <br> |