Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/29

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＜Result＞<br />
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[[Image:Matsuura_2010-08-29_21hr_14min.tif‎]]
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[[Image:Matsuura 2010-08-06 20hr 02min (6).JPG‎]]

Revision as of 06:19, 28 September 2010


Contents

2010/08/29

1：PCR

＜Member＞
Mariko, nito


＜Sample＞
・E-coli (having BBa_I13521 plasmid)

On 8/28, we used incorrect plasmid, so we had PCR again.


＜Protocol＞
See Protocol 2

・Tube (temperature in annealing…HH/H/L/LL)
3. mRfp~terminator (69.0℃/67.5/66.0/63.0)
7. Terminator (69.0/67.5/66.0/63.0)

Total 8 tubes.


2：DNA extraction

＜Member＞
nito


＜Sample＞
・PCR productions


＜Protocol＞
SeeProtocol 4


3：DNA concentration measurement

＜Member＞
nito


＜Sample＞
・PCR products


＜Protocol＞

  1. Add 2µl of TE and 2µl of sample into the tube, and mix it gently.
  2. Fall in drops 1 on the measuring machine.


＜Result＞

DNA concentration
concentration A320 A260/A280 A260/A230
3HH 39.0 0.500 1.814 0.161
3H 23.5 0.051 1.858 0.091
3L 39.5 0.400 1.829 0.272
3LL 17.0 0.189 1.868 0.086
7HH 28.5 0.070 1.894 0.099
7H 27.0 0.045 1.829 0.046
7L 38.0 0.057 1.900 0.175
7LL 12.2 0.000 1.885 0.299


4：Electrophoresis

＜Member＞
Mariko, nito


＜Sample＞
・PCR products


＜Protocol＞
SeeProtocol 8


＜Result＞
Matsuura 2010-08-06 20hr 02min (6).JPG

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