Team:Lethbridge/Notebook/Lab Work/September
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<b>Results:</b> TBD <br> | <b>Results:</b> TBD <br> | ||
<b>Follow-up:</b> TBD<br> | <b>Follow-up:</b> TBD<br> | ||
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<b>Objective:</b> Create glycerol stocks of J04450 in pSB1A3, pSB1K3 and pSB1T3 for use in RFP-BioBrick Assembly.<br> | <b>Objective:</b> Create glycerol stocks of J04450 in pSB1A3, pSB1K3 and pSB1T3 for use in RFP-BioBrick Assembly.<br> | ||
<b>Method:</b> Transform into Subcloning Efficiency Competent DH5α Cells (Invitrogen)<br> | <b>Method:</b> Transform into Subcloning Efficiency Competent DH5α Cells (Invitrogen)<br> | ||
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*Generate Glycerol Stocks | *Generate Glycerol Stocks | ||
*Generate Plasmid DNA via Maxiprep | *Generate Plasmid DNA via Maxiprep | ||
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<b>Objective:</b> Create glycerol stocks of received synthesized (Mr. Gene) signal peptides. | <b>Objective:</b> Create glycerol stocks of received synthesized (Mr. Gene) signal peptides. | ||
<b>Method:</b> Transform into Subcloning Efficiency Competent DH5α Cells (Invitrogen) plasmid DNA containing the following BioBricks: | <b>Method:</b> Transform into Subcloning Efficiency Competent DH5α Cells (Invitrogen) plasmid DNA containing the following BioBricks: |