Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/17

From 2010.igem.org

(Difference between revisions)
(→Experiment:Electrophoreses of PCR productions)
Line 14: Line 14:
'''Procedure'''
'''Procedure'''
-
#set agarose gel and add TAE buffer in gel box.
+
#Set agarose gel and add TAE buffer in electrophoresis tank.
-
#mix Loading Buffer and pSB1C3 and then put in well them(marker sets another well).
+
#Mix Loading Buffer and pSB1C3 ,then put them in well (marker sets another well).
-
#load DNA at 100V for two third of entire (about 15 minutes).
+
#Load DNA at 100V for two thirds of total volume (about 15 minutes).
-
#image the consequence of electrophoreses.
+
#Image the consequence of electrophoresis.
'''Result'''
'''Result'''

Revision as of 06:35, 21 September 2010


2010/8/17 Tuesday (watachin)

Experiment:Electrophoreses of PCR productions

Member
NEX and watachin

Materials

  • pSB1A3(25ng/μl) 22μl
  • 10*Loading buffer 2.2μl
  • DNA Marker 5μl
  • 1*TAE buffer
  • 1% agarose gel

Procedure

  1. Set agarose gel and add TAE buffer in electrophoresis tank.
  2. Mix Loading Buffer and pSB1C3 ,then put them in well (marker sets another well).
  3. Load DNA at 100V for two thirds of total volume (about 15 minutes).
  4. Image the consequence of electrophoresis.

Result

2010-08-17-ef.jpg

failure

Plasmid concentration was too low.

Experiment:Transformation of pSB1A3

Member
NEX and watachin

Material

  • pSB1A3 1μl
  • competent cell DH5α 50μl
  • LB + amp plate

Procedure

  1. mix pSB1A3 and DH5α
  2. on ice (30min)
  3. heat shock 42℃ 45sec
  4. on ice (2min)
  5. inoculate this onto plate
  6. incubate cells at 37℃
Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/08/17"