Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/11

From 2010.igem.org

(Difference between revisions)
(→Experiment:Grow up a culture of A.xylinus)
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==2010/8/11 Wednesday (Naoto)==
==2010/8/11 Wednesday (Naoto)==
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===Experiment:Grow up a culture of ''A.xylinus''===
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===Experiment:Grow up a culture of ''A.xylinum''===
'''Member'''<br />
'''Member'''<br />
easily and naoto
easily and naoto
'''Materials'''
'''Materials'''
 +
*''A.xylinum'' JCM strain 7644
*''A.xylinum'' JCM strain 7644
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*media for A.xylinus(Open Wet Ware recommended)  
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*media for A.xylinum(Open Wet Ware recommended)  
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*media for A.xylinus(JCM recommended) 100ml
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*media for A.xylinum(JCM recommended) 100ml
  glucoce 10g
  glucoce 10g
  yeast extract 1g
  yeast extract 1g
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  DW 100ml
  DW 100ml
  (add agar 1.5g for plates)
  (add agar 1.5g for plates)
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*ｒecovery solution for ''A.xylinus'' 100ml
+
*ｒecovery solution for ''A.xylinum'' 100ml
  peptone 0.5g
  peptone 0.5g
  yeast extract 0.3g
  yeast extract 0.3g
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'''Procedure'''
'''Procedure'''
 +
#prepared media (plates of Open Wet Ware recommended media were made yesterday)
#prepared media (plates of Open Wet Ware recommended media were made yesterday)
#autoclaved to sterilize media(121°C　20minute)  
#autoclaved to sterilize media(121°C　20minute)  
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#added ｒecovery solution to an ampule which had ''A.xylinus''
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#added ｒecovery solution to an ampule which had ''A.xylinum''
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#streaked ''A.xylinus'' onto plates or in media
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#streaked ''A.xylinum'' onto plates or in media
#incubated at 26°C
#incubated at 26°C

Revision as of 15:14, 16 September 2010


2010/8/11 Wednesday (Naoto)

Experiment:Grow up a culture of A.xylinum

Member
easily and naoto

Materials

  • A.xylinum JCM strain 7644
  • media for A.xylinum(Open Wet Ware recommended)
  • media for A.xylinum(JCM recommended) 100ml 
glucoce 10g
yeast extract 1g
CaCO3 3g
DW 100ml
(add agar 1.5g for plates)
  • ｒecovery solution for A.xylinum 100ml 
peptone 0.5g
yeast extract 0.3g
DW 100ml

Procedure

  1. prepared media (plates of Open Wet Ware recommended media were made yesterday)
  2. autoclaved to sterilize media(121°C　20minute)
  3. added ｒecovery solution to an ampule which had A.xylinum
  4. streaked A.xylinum onto plates or in media
  5. incubated at 26°C

Experiment:Grow up a culture of E.coli

Member
NEX,Bambi75 and watachin

Materials
LB plates

Procedure

  1. streaked E.coli onto plates
  2. incubated at 37°C
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