Gel extraction of amplified plasmid pSB1C3 for rocF

Aim

The aim of this experiment is to perform gel extraction of the band containing the amplified fragment of the plasmid vector pSB1C3.

Materials and Protocol

Please refer to: Gel extraction.

Result

For plasmid vector pSB1C3, we used 1.5% agarose gel and run the gel on 75 Volts for better resolution. We used 1Kb DNA ladder (Promega) for the gel containing plasmid vector.

Table 2: Table represents the size of the plasmid vector pSB1C3 represented as bands on the gel.

Discussion

We found bands of appropriate size and cut it out and placed the part of the gel in a 2ml eppendorf tube.

Conclusion

Gel electrophoresis was successful and we did gel extraction of the fragment containing plasmid pSB1C3.