Team:SDU-Denmark/labnotes6
From 2010.igem.org
(Difference between revisions)
(→Group: Retinal) |
(→Lab notes (16/9 - 22/9)) |
||
Line 9: | Line 9: | ||
== Group: Retinal == | == Group: Retinal == | ||
- | === | + | ==== ON of Top 10 E. coli cells with POT2 with NinaB ==== |
- | + | Date: 16/8<br> | |
+ | Done by: Marie & Tommy<br> | ||
+ | Methods: | ||
+ | protocos: | ||
+ | <br><br> | ||
+ | Notes:<br> | ||
+ | 100 mL ON culture was made, cells were grown at 37 C in LB media with Chloramphenicol. | ||
+ | ==== ON of Top 10 E. coli cells with POT2 with NinaB ==== | ||
+ | Date: 17/8<br> | ||
+ | Done by: Marie & Tommy<br> | ||
+ | Methods: Miniprep, Restriction digest, gel<br> | ||
+ | Protocols: MP1.2 [https://2010.igem.org/Team:SDU-Denmark/protocols#MP1.2], RD1.1 [https://2010.igem.org/Team:SDU-Denmark/protocols#RD1.1]. | ||
+ | <br><br> | ||
+ | Notes:<br> | ||
+ | 50 mL of elution buffer was used. DNA concentrations after pooling were measured on NanoDrop to 206,2 ng/microL. | ||
+ | gel was run on produckt, showing recent miniprep compared to old miniprep produckt, showing uneven lengths. subsequent was performed.<br> | ||
+ | [[Image:Team-sdu-denmark-Minipreb_product_of_POT2_with_NinaB_1,5_gel_red_loader__-4.jpg | 400px ]] | ||
+ | ==== Restriction digest on miniprep produckt (w. EcoRI) ==== | ||
+ | Date: 17/8<br> | ||
+ | Done by: Marie & Tommy<br> | ||
+ | Methods: Restriction digest, gel<br> | ||
+ | Protocols: MP1.2 [https://2010.igem.org/Team:SDU-Denmark/protocols#MP1.2], gel. | ||
+ | <br><br> | ||
+ | Notes:<br> | ||
+ | Due to the lack of old sample, restriction digest was performed using only 3,5 microL of miniprep produckt. 1,5 microL of H2O was added insted. EcoRI was used<br> | ||
+ | The digest mix was incubated for 10 min at 37 C. A gel was run showing uncut new, cut new, uncut old and cut old miniprep product.<br> | ||
+ | [[Image:Team-sdu-denmark-Minipreb_product_of_POT2_with_NinaB_1,5_gel_red_loader__-5.jpg |400px ]] |
Revision as of 09:36, 1 September 2010