Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/11
From 2010.igem.org
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===Experiment:Grow up a culture of ''A.xylinus''=== | ===Experiment:Grow up a culture of ''A.xylinus''=== | ||
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'''Materials''' | '''Materials''' |
Revision as of 09:48, 17 August 2010
2010/8/11 Wednesday (Naoto)
Experiment:Grow up a culture of A.xylinus
Member
easily and naoto
Materials
- A.xylinum JCM strain 7644
- media for A.xylinus(Open Wet Ware recommended)
- media for A.xylinus(JCM recommended) 100ml
glucoce 10g yeast extract 1g CaCO3 3g DW 100ml (add agar 1.5g for plates)
- recovery solution for A.xylinus 100ml
peptone 0.5g yeast extract 0.3g DW 100ml
Procedure
- prepared media (plates of Open Wet Ware recommended media were made yesterday)
- autoclaved to sterilize media(121°C 20minute)
- added recovery solution to an ampule which had A.xylinus
- streaked A.xylinus onto plates or in media
- incubated at 26°C
Experiment:Grow up a culture of E.coli
Member
NEX,Bambi75 and watachin
Materials
LB plates
Procedure
- streaked E.coli onto plates
- incubated at 37°C