From 2010.igem.org

Revision as of 12:52, 15 August 2010

2010/08/06

Experiment1：Making LB plate (20ml×20)

＜Member＞Mariko, tomy, easily, naoto, nito, Taka, watachin

＜Materials＞
・Yeast extract 2.0g

・Peptone 2.0g

・Glucose 40.0g

・NaCl 2.0g

・Agerose 6.0g

・Ampcillim 0.4mg

・Dropped water 400ml

＜Protocol＞
1 Prepared 400ml solution to contain Yeast extract 2.0g, Peptone 2.0g, Glucose 40.0g, NaCl 2.0g and Agerose 6.0g.

2 Divided up 400ml solution into two Erlenmeyer flasks, and sealed with aluminum. Afterwards , started autoclave(121℃,20min)

3 After finished autoclave, in cleanbench, added 0.2ml Ampcillm to each of the solutions. Afterwards, divided solutions into twenty Petri dishes.

4 Cooled and dried divided solutions in Petri dishes. After Cooling and drying well, kept these in refrigerator.

Experiment2；Scattering E-coli (having BioBrick plasmid) on LB plate

＜Member＞tomy, easily, naoto, nito

＜Materials＞

・LB plate×4

・E-coli (having BBa_I1732901 plasmid)

・E-coli (having BBa_K208017 plasmid)

<Protocol＞

1 Sterilized Inoculation loop by flame. After Sterilization, pick up E-coli by Inoculation loop、and then scatter on LB plate.

2 Incubated these plate at 37℃.