Team:Newcastle/13 August 2010
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==Aims== | ==Aims== | ||
- | + | We plan to select three ''spaIFEG'' gene (part 3) PCR tubes, using Tms 51, 56 and 61 (we had confirmed it successful from yesterday's gel electrophoresis). Gel extraction will then be performed, along with Plasmid Vector (part 1), Promoter & RBS (part 2) and Double terminator (part 4). Finally, NanoDrop will be performed. | |
==Materials and protocol== | ==Materials and protocol== | ||
- | Please refer to the [[Team:Newcastle/Gel_electrophoresis| gel electrophoresis]] | + | Please refer to the [[Team:Newcastle/Gel_electrophoresis| gel electrophoresis]], [[Team:Newcastle/Gel_extraction| gel extraction]] and [[Team:Newcastle/NanoDrop_Spectrophotometer| NanoDrop]] protocols. |
==Results== | ==Results== |
Revision as of 14:54, 13 August 2010
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Contents |
rocF BioBrick miniprep
rocF BioBrick nanodrop
rocF BioBrick gel electrophoresis
Expect band to be 3195bp.
Subtilin Immunity BioBrick
Aims
We plan to select three spaIFEG gene (part 3) PCR tubes, using Tms 51, 56 and 61 (we had confirmed it successful from yesterday's gel electrophoresis). Gel extraction will then be performed, along with Plasmid Vector (part 1), Promoter & RBS (part 2) and Double terminator (part 4). Finally, NanoDrop will be performed.
Materials and protocol
Please refer to the gel electrophoresis, gel extraction and NanoDrop protocols.
Results
Discussion
Conclusion
Go back to our main Lab book page
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