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Team:Newcastle/5 July 2010
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| - | === | + | '''5 July 2010''' |
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| + | =LacI BioBrick Construction= | ||
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| + | ==Aims== | ||
*To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP). | *To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP). | ||
| - | + | ==Materials== | |
* Refrigerated [[Team:Newcastle/1_July_2010#LacI_BioBrick_Construction|transformation plates]]. | * Refrigerated [[Team:Newcastle/1_July_2010#LacI_BioBrick_Construction|transformation plates]]. | ||
| - | + | ==Protocol== | |
* Set up a [[Team:Newcastle/Growing_an_overnight_cultures|overnight culture]] of colonies transformed with pMutin4 and pSB1AT3. | * Set up a [[Team:Newcastle/Growing_an_overnight_cultures|overnight culture]] of colonies transformed with pMutin4 and pSB1AT3. | ||
| - | + | ==Inference== | |
*Transformed ''E. coli'' DH5α grown up overnight to create a large stock of plasmid ready for extraction. | *Transformed ''E. coli'' DH5α grown up overnight to create a large stock of plasmid ready for extraction. | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Revision as of 10:49, 11 August 2010
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5 July 2010
Contents |
LacI BioBrick Construction
Aims
- To use PCR to extract lacI (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).
Materials
- Refrigerated transformation plates.
Protocol
- Set up a overnight culture of colonies transformed with pMutin4 and pSB1AT3.
Inference
- Transformed E. coli DH5α grown up overnight to create a large stock of plasmid ready for extraction.
   
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