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Team:Calgary/10 August 2010
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| + | Today I ligated the products of pRFP that I had done a restriction digest and antarctic phosphatase on. And also transformed and plated them. I also did a gradient PCR with the remaining amount of pRFP and am doing a colony PCR of the plates where pRFP was gained from as well as the growth from one of the plates from a previous plasmid switch. I ran all the PCR products on a gel and used the information from the colony PCR to do overnights as well as PCR purify the gradient PCR. | ||
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Revision as of 18:25, 10 August 2010
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Tuesday August 10, 2010
Raida
Jeremy
Today I ligated the products of pRFP that I had done a restriction digest and antarctic phosphatase on. And also transformed and plated them. I also did a gradient PCR with the remaining amount of pRFP and am doing a colony PCR of the plates where pRFP was gained from as well as the growth from one of the plates from a previous plasmid switch. I ran all the PCR products on a gel and used the information from the colony PCR to do overnights as well as PCR purify the gradient PCR.
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