Team:Calgary/2 August 2010
From 2010.igem.org
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Obtained no colonies from yesterday's plasmid switch. Decided to re-ligate the digested insert and vector from yesterday, transform and plate. | Obtained no colonies from yesterday's plasmid switch. Decided to re-ligate the digested insert and vector from yesterday, transform and plate. | ||
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+ | <u>Patrick</u> | ||
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+ | Didn't come in for too long today, but I looked into NURBs modelling and IPR Rendering for Maya. | ||
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Revision as of 22:01, 6 August 2010
![University of Calgary iGEM Team 2010](http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Header4.png)
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Monday August 2, 2010
Heritage Day: A Day off!! ...For most of us.
Jeremy
Set up a restriction digest of pRFP without PCR purification and left overnight. This was cut with EcoRI and SpeI. Since the sample had salts from PCR, concentration was not measured therefore a estimation of around 5.5uL to make the total concentration of the sample 10 uL. A PCR purification was made using older sample however even combining all the wells making the DNA volume in the tube 100uL, the concentrations were in their negatives. PCR purification will not be pursued further. Attempts will be made to plasmid switch PCR product without purification.
Dev
Obtained no colonies from yesterday's plasmid switch. Decided to re-ligate the digested insert and vector from yesterday, transform and plate.
Patrick
Didn't come in for too long today, but I looked into NURBs modelling and IPR Rendering for Maya.
No notebook page exists for this date. Sorry!