Monday August 2, 2010

Heritage Day: A Day off!! ...For most of us.

Jeremy

Set up a restriction digest of pRFP without PCR purification and left overnight. This was cut with EcoRI and SpeI. Since the sample had salts from PCR, concentration was not measured therefore a estimation of around 5.5uL to make the total concentration of the sample 10 uL. A PCR purification was made using older sample however even combining all the wells making the DNA volume in the tube 100uL, the concentrations were in their negatives. PCR purification will not be pursued further. Attempts will be made to plasmid switch PCR product without purification.

Dev

Obtained no colonies from yesterday's plasmid switch. Decided to re-ligate the digested insert and vector from yesterday, transform and plate.