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Team:Newcastle/27 July 2010
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===Protocol=== | ===Protocol=== | ||
* Please refer to the [[Team:Newcastle/DNA extraction| DNA extraction of ''B. subtilis'']] page | * Please refer to the [[Team:Newcastle/DNA extraction| DNA extraction of ''B. subtilis'']] page | ||
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===Discussion=== | ===Discussion=== | ||
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===Conclusion=== | ===Conclusion=== | ||
The experiment was a success! The content and purity of the extracted DNA will be checked by using PCR on 28th July, 2010. | The experiment was a success! The content and purity of the extracted DNA will be checked by using PCR on 28th July, 2010. | ||
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==Gel Electrophoresis== | ==Gel Electrophoresis== | ||
Revision as of 10:45, 3 August 2010
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Contents |
Genomic DNA extraction experiment
Aims
The aim of today's experiment is to extract genomic DNA from both B. subtilis strains 168 and 3610. The genes necessary for the swarming biobrick and rocF biobrick will then hopefully be obtained from the genomic DNA using...
Protocol
- Please refer to the DNA extraction of B. subtilis page
Discussion
At the end of the DNA precipitation step, we did observe a small white pellet in all the eppendorf tubes.
Conclusion
The experiment was a success! The content and purity of the extracted DNA will be checked by using PCR on 28th July, 2010.
Gel Electrophoresis
Protocol
- Please refer to the gel electrophoresis page for full protocol.
Discussion
- Unfortunately no bands were observed on the gel.
Conclusion
As no bands were observed on the gel, the PCR did not work. This could be due to a number of reasons: It could be due to the DNA not being extracted or that we might have used the wrong primers. It could also be caused by the small size of the fragment that we amplified which ran off the gel because we ran the gel for too long.
   
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