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Team:Newcastle/27 July 2010
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| - | The PCR did not work. It could be due to the DNA not being extracted or we used the wrong primers. It could also be caused by the small size of the fragment that we amplified which ran off the gel because we ran the gel for too long. | + | The PCR did not work. It could be due to the DNA not being extracted or that we might have used the wrong primers. It could also be caused by the small size of the fragment that we amplified which ran off the gel because we ran the gel for too long. |
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Revision as of 13:48, 27 July 2010
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Contents |
Genomic DNA extraction experiment
Aim
The aim of today's experiment is to extract genomic DNA from Bacillus subtilis strain 3610 and 168,genes from which will be needed for the swarming biobrick and rocF biobrick.
Procedure
- Please refer to DNA extraction of Bacillus subtilis
Discussion
At the end of the DNA precipitation step, we did observe a small white pellet in all the eppendorf tubes.
Conclusion
Th experiment was successful and we would check the content and the purity of the extracted DNA by using PCR on 28th July, 2010.
Gel Electrophoresis
Materials
- 1% Agarose TAE
- SafeView
- TAE Buffer
Protocol
- Please refer to Gel Electrophoresis
Discussion
- No band was observed on the gel.
Conclusion
The PCR did not work. It could be due to the DNA not being extracted or that we might have used the wrong primers. It could also be caused by the small size of the fragment that we amplified which ran off the gel because we ran the gel for too long.
   
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