Team:Newcastle/22 July 2010

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Revision as of 13:10, 27 July 2010

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 ===Aim of this experiment===
 The aim of this experiment is to determine whether ''B. subtilis'' 168 is able to take up external arginine.
-===Materials Required===
-* Plate consisting of ''Bacillus subtilis'' 168 colonies.
-* Flame (streaking) Loop
-* LB media consisting arginine and ampicillin
-* Auto pipette
-* Bursen Burner
-* Universal Tube
 ===Procedure===
-* Perform the experiment using aseptic technique.
+* Please refer to [[Team:Newcastle/Arginine test| Arginine test]]
-* Transfer ''B. subtilis'' 168 colonies into universal tubes containing 5 ml of LB media and allowed to grow overnight at 37° C.
-* Transfer 1 ml of the overnight culture to another universal tube containing 4 ml of the following media:
-#Control (1) - LB media
-#Control (2) - LB media with 10 mM of arginine
-#Control (3) - LB media plus ''B. subtilis'' 168
-#Test (1) - LB media with 10 mM of arginine plus ''B. subtilis'' 168
-#Test (2) - LB media with 10 mM of arginine plus ''B. subtilis'' 168
-* Incubate the culture at 37° C with shaking.
-* Record the pH at every 30 min interval.Use 20 ul of the culture and measure the pH using the pH measuring stick.
 ===Expected results===