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Team:Newcastle/27 July 2010
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The aim of today's experiment is to extract genomic DNA from ''Bacillus subtilis'' strain 3610 and 168,genes from which will be needed for the swarming biobrick and ''rocF'' biobrick. | The aim of today's experiment is to extract genomic DNA from ''Bacillus subtilis'' strain 3610 and 168,genes from which will be needed for the swarming biobrick and ''rocF'' biobrick. | ||
Revision as of 13:03, 27 July 2010
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Contents |
Genomic DNA extraction experiment
Aim
The aim of today's experiment is to extract genomic DNA from Bacillus subtilis strain 3610 and 168,genes from which will be needed for the swarming biobrick and rocF biobrick.
Procedure
- Please refer to DNA extraction of Bacillus subtilis
Discussion
At the end of the DNA precipitation step, we did observe a small white pellet in all the eppendorf tubes.
Conclusion
Th experiment was successful and we would check the content and the purity of the extracted DNA by using PCR on 28th July, 2010.
Gel Electrophoresis
Protocol
- Please refer to Gel Electrophoresis
Materials
- 50ml of 1% Agarose TAE
- 5µl SafeView
- TAE Buffer
Discussion
   
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