Team:Newcastle/2 July 2010

From 2010.igem.org

(Difference between revisions)
(LacI BioBrick Construction)
Line 24: Line 24:
===Conclusion===  
===Conclusion===  
The negative control did not turn pink-red color, therefore indicating that no contamination had occurred.
The negative control did not turn pink-red color, therefore indicating that no contamination had occurred.
-
 
-
 
-
 
==Competent ''E. coli'' Production==
==Competent ''E. coli'' Production==

Revision as of 10:31, 27 July 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Contents

Urease Test

Aims

The aim of this experiment was to determine if Bacillus subtilis 168 is able to produce urease and degrade urea.

Materials

The experiment was performed on 01.07.10. For the materials used, please see 01.07.10 lab notebook.

Protocol

The experiment was performed on 01.07.10. For the experimental protocol, please see 01.07.10 lab notebook.

Results

|Newcastle urease test 020710.png

Figure 1: Urease test results

  1. Negative control - No color change (Orange color)
  2. Test 1 (Duplicate) - Pink-red color
  3. Test 2 (Duplicate) - Pink-red color

Discussion

B. subtilis 168 is able to produce urease, therefore, urea which is the substrate and can be found in the agar was degraded, which results in ammonia building. The ammonia makes the media alkaline and therefore the indicator phenol red will change from orange color to pink-red color.

Conclusion

The negative control did not turn pink-red color, therefore indicating that no contamination had occurred.

Competent E. coli Production

Aims

  • To make a stock of competent E. coli (DH5alpha strain) ready for transformation.

Materials

  • E. coli DH5alpha strain

Protocol

Inference

  • Grow up a stock of E.coli DH5alpha strain ready to be made competent



Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon