Team:Newcastle/2 July 2010

B. subtilis 168 is able to produce urease, therefore, urea which is the substrate and can be found in the agar was degraded, which results in ammonia building. The ammonia makes the media alkaline and therefore the indicator phenol red will change from orange color to pink-red color.

Conclusion

The negative control did not turn pink-red color, therefore indicating that no contamination had occurred.

Competent E. coli Production

Aims

To make a stock of competent E. coli (DH5alpha strain) ready for transformation.

Materials

E. coli DH5alpha strain

Protocol

Set up liquid culture of E. coli DH5alpha

Inference

Grow up a stock of E.coli DH5alpha strain ready to be made competent

Produce competent E. coli (DH5alpha strain)

@@ Line 26: / Line 26: @@
-==LacI BioBrick Construction==
-===Aims===
-*To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).
-===Materials===
-*Competent ''E. coli'' (DH5alpha strain)
-*pMutin4 plasmid
-*pSB1AT3 plasmid
-===Protocol===
-* [[TeamNewcastleTransformation of E. coli|Transformation of ''E. coli'']] DH5alpha with pMutin4 and pSB1AT3 separately.
-===Inference===
-*''Ecoli'' DH5alpha is used as a bioreactor to replicate our plasmids to create a large stock ready for extraction from cells.

Team:Newcastle/2 July 2010

From 2010.igem.org

Revision as of 10:30, 27 July 2010

Contents

Urease Test

Aims

Materials

Protocol

Results

Discussion

Conclusion

Competent E. coli Production

Aims

Materials

Protocol

Inference