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Team:EPF Lausanne/Calendar/0716
From 2010.igem.org
(Difference between revisions)
(New page: {{EPFL_2010_calendar_day}} <div CLASS="EPFL_calendar_content"> = 16 July 2010 = Empty yet but it will be filled soon, don't worry ;) </div> <html> <table style="width: 100%"> <tr> <...) |
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| - | + | *ligation Asaia origin BB + vector | |
| + | *ligation Asaia origin BB + Kan + vector | ||
| + | *ligation Asaia origin BB + Amp + vector | ||
| + | *ligation Asaia origin BB + Cm + vector | ||
| + | *ligation Asaia origin BB + Tet + vector | ||
| + | *Extraction of the OD curve data ([https://2010.igem.org/Image:Results_OD_asaia.xls values]) | ||
| + | *Replating Asaia on plates with and without kanamicin in order to recover the WT Asaia (only WT do not grow on kanamicin) | ||
| + | *Feeding and infection of Drosophila (WT and immunodeficient redish mutants) with bacteria Ecc15 and Asaia in Prof. Lemaitre Lab | ||
| + | *Fluorescence microscopy -> presence of fluorescence in the body/gut as expected | ||
| + | *Transformation of E-Coli with the ligation products and plating the transformed bacteria | ||
</div> | </div> | ||
Latest revision as of 18:52, 23 July 2010
16 July 2010
- ligation Asaia origin BB + vector
- ligation Asaia origin BB + Kan + vector
- ligation Asaia origin BB + Amp + vector
- ligation Asaia origin BB + Cm + vector
- ligation Asaia origin BB + Tet + vector
- Extraction of the OD curve data (values)
- Replating Asaia on plates with and without kanamicin in order to recover the WT Asaia (only WT do not grow on kanamicin)
- Feeding and infection of Drosophila (WT and immunodeficient redish mutants) with bacteria Ecc15 and Asaia in Prof. Lemaitre Lab
- Fluorescence microscopy -> presence of fluorescence in the body/gut as expected
- Transformation of E-Coli with the ligation products and plating the transformed bacteria
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