16 July 2010

• ligation Asaia origin BB + vector
• ligation Asaia origin BB + Kan + vector
• ligation Asaia origin BB + Amp + vector
• ligation Asaia origin BB + Cm + vector
• ligation Asaia origin BB + Tet + vector
• Extraction of the OD curve data (values)
• Replating Asaia on plates with and without kanamicin in order to recover the WT Asaia (only WT do not grow on kanamicin)
• Feeding and infection of Drosophila (WT and immunodeficient redish mutants) with bacteria Ecc15 and Asaia in Prof. Lemaitre Lab
• Fluorescence microscopy -> presence of fluorescence in the body/gut as expected
• Transformation of E-Coli with the ligation products and plating the transformed bacteria