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Team:Brown/Notebook/June24
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(Difference between revisions)
(New page: ==Thursday, June 24 2010== Plated both immotile strains (DFB22, DFB 201) and three inducibly motile strains (pDB28, pDB101, pDB102) all in null media, amicillin + IPTG, and chloramphenico...) |
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==Thursday, June 24 2010== | ==Thursday, June 24 2010== | ||
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{| border="1" | {| border="1" | ||
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!Strain | !Strain | ||
!null plate | !null plate | ||
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!Chlor+arab | !Chlor+arab | ||
|- | |- | ||
| - | DFB 22||immotile growth||no growth||no growth | + | |DFB 22||immotile growth||no growth||no growth |
|- | |- | ||
| - | DFB 201||immotile growth||no growth||no growth | + | |DFB 201||immotile growth||no growth||no growth |
|- | |- | ||
| - | DFB 22 w/ pDB28||immotile growth||motile growth||no growth | + | |DFB 22 w/ pDB28||immotile growth||motile growth||no growth |
|- | |- | ||
| - | DFB 201 w/ pDB101||immotile growth||no growth||motile growth | + | |DFB 201 w/ pDB101||immotile growth||no growth||motile growth |
|- | |- | ||
| - | DFB 201 w/ pDB102||immotile growth||motile growth||no growth | + | |DFB 201 w/ pDB102||immotile growth||motile growth||no growth |
|} | |} | ||
| - | MRS Plates | + | ===MRS Plates=== |
| + | Notation for chlor is 2 stripes. | ||
Total – 500 mL | Total – 500 mL | ||
| - | 250 mL | + | *250 mL Null plates |
| - | 250 mL | + | *250 mL Chloramphenicol plates at 15 µg/mL |
| - | + | Total = 3750 µg (3.75 mg) | |
| - | + | ||
| + | Stock – 34 mg/mL | ||
Latest revision as of 18:57, 19 July 2010
Thursday, June 24 2010
Plated both immotile strains (DFB22, DFB 201) and three inducibly motile strains (pDB28, pDB101, pDB102) all in null media, amicillin + IPTG, and chloramphenicol + arabinose for a total of 6 x 3 = 18 plates and stabs (including stabs).
The expected results are:
| Strain | null plate | amp+IPTG | Chlor+arab |
|---|---|---|---|
| DFB 22 | immotile growth | no growth | no growth |
| DFB 201 | immotile growth | no growth | no growth |
| DFB 22 w/ pDB28 | immotile growth | motile growth | no growth |
| DFB 201 w/ pDB101 | immotile growth | no growth | motile growth |
| DFB 201 w/ pDB102 | immotile growth | motile growth | no growth |
MRS Plates
Notation for chlor is 2 stripes. Total – 500 mL
- 250 mL Null plates
- 250 mL Chloramphenicol plates at 15 µg/mL
Total = 3750 µg (3.75 mg)
Stock – 34 mg/mL
