Team:Queens-Canada/14 October 2010

From 2010.igem.org

(Difference between revisions)
 
Line 22: Line 22:
Used 0.3 g agarose in 50 mL TBE. Gel run for 40 mins at 100 V.
Used 0.3 g agarose in 50 mL TBE. Gel run for 40 mins at 100 V.
 +
 +
<h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3>
 +
Hao
 +
 +
'''pStr-220'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3
 +
 +
Co-injected construct with pRF4 marker.
 +
 +
'''pStr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pHsp-3'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pFlp-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3
 +
 +
Examined transgenic worms under confocal microscope; no fluorescence detected in target neuron.
{{:Team:Queens-Canada/foot}}
{{:Team:Queens-Canada/foot}}

Latest revision as of 03:44, 28 October 2010

October 14, 2010

Heat Shock

Steve

pStr-1::eCFP::unc-54 3'UTR::1C3 from 07/10/10

Liquid Culture

Steve

pFlp-1::eCFP::unc-54 3'UTR::1C3, pSra-10::eCFP::unc-54 3'UTR::1C3, ChR2::eYFP::unc-54 3'UTR::1C3, pHsp-3::eCFP::unc-54 3'UTR::1C3, pOsm-10::eCFP::unc-54 3'UTR::1C3, pOdr-1::eCFP::unc-54 3'UTR::1C3, eYFP::unc-54 3'UTR::1C3, mCherry::unc-54 3'UTR::1C3

Digestion-Ligation

Steve

pGpd-2, pOdr-10, pStr-220, pOdr-10, pSip-1, eCFP::unc-54 3'UTR::1C3

Gel Electrophoresis

Steve

Ligation products from 14/10/10

Used 0.3 g agarose in 50 mL TBE. Gel run for 40 mins at 100 V.

Microinjection

Hao

pStr-220::eCFP::unc-54 3'UTR::1A3

Co-injected construct with pRF4 marker.

pStr-1::eCFP::unc-54 3'UTR::1A3, pHsp-3::eCFP::unc-54 3'UTR::1A3, pFlp-1::eCFP::unc-54 3'UTR::1A3

Examined transgenic worms under confocal microscope; no fluorescence detected in target neuron.