Team:Queens-Canada/14 October 2010

Heat Shock

Steve

pStr-1::eCFP::unc-54 3'UTR::1C3 from 07/10/10

Liquid Culture

Steve

pFlp-1::eCFP::unc-54 3'UTR::1C3, pSra-10::eCFP::unc-54 3'UTR::1C3, ChR2::eYFP::unc-54 3'UTR::1C3, pHsp-3::eCFP::unc-54 3'UTR::1C3, pOsm-10::eCFP::unc-54 3'UTR::1C3, pOdr-1::eCFP::unc-54 3'UTR::1C3, eYFP::unc-54 3'UTR::1C3, mCherry::unc-54 3'UTR::1C3

Digestion-Ligation

Steve

pGpd-2, pOdr-10, pStr-220, pOdr-10, pSip-1, eCFP::unc-54 3'UTR::1C3

Gel Electrophoresis

Steve

Ligation products from 14/10/10

Used 0.3 g agarose in 50 mL TBE. Gel run for 40 mins at 100 V.

Microinjection

Hao

pStr-220::eCFP::unc-54 3'UTR::1A3

Co-injected construct with pRF4 marker.

pStr-1::eCFP::unc-54 3'UTR::1A3, pHsp-3::eCFP::unc-54 3'UTR::1A3, pFlp-1::eCFP::unc-54 3'UTR::1A3

Examined transgenic worms under confocal microscope; no fluorescence detected in target neuron.