Team:Queens-Canada/7 October 2010

Digestion-Ligation

Thai and Hao

eCFP::unc-54 3'UTR, pOdr-10::eCFP::unc-54 3'UTR, pGpd-2::eCFP::unc-54 3'UTR, pSra-10::eCFP::unc-54 3'UTR, pFlp-1::eCFP::unc-54 3'UTR, ChR2::eCFP::unc-54 3'UTR, pHsp-3::eCFP::unc-54 3'UTR, pOsm-10::eCFP::unc-54 3'UTR, eYFP::eCFP::unc-54 3'UTR, mCherry::eCFP::unc-54 3'UTR, pStr-220::eCFP::unc-54 3'UTR, pSip-1::eCFP::unc-54 3'UTR, pOdr-1::eCFP::unc-54 3'UTR, pStr-1::eCFP::unc-54 3'UTR, pSB1C3

Backbone treated with CIAP for 30 mins.

Microinjection

Hao

pStr-1::eCFP::unc-54 3'UTR::1A3, pHsp-3::eCFP::unc-54 3'UTR::1A3, pFlp-1::eCFP::unc-54 3'UTR::1A3

Co-injected each construct with pRF4 marker.