Team:Chiba/Project

From 2010.igem.org

(Difference between revisions)
(→Overall project)
 
(240 intermediate revisions not shown)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
+
<!-- ---------------------------------------------------------
-
 
+
--------------------------------------------------------------
 +
下の方に「ここから下を編集」というところがあります
 +
そっちを編集してください
 +
--------------------------------------------------------------
 +
------------------------------------------------------------ -->
 +
<html><link rel="stylesheet" href="https://2010.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html>
<html>
<html>
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
+
<style type="text/css">
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
+
#top-section {
-
This is a template page. READ THESE INSTRUCTIONS.
+
    height: 0px;
-
</div>
+
    margin-top: -80px;
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
+
    margin-left: auto;
-
You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
+
    margin-right: auto;
-
</div>
+
    margin-bottom: 0 !important;
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
+
    margin-bottom: 0px;
-
You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
+
    padding:0;
-
</div>
+
    border: 0;
-
</div>
+
    }
-
</html>
+
p{margin:10px 20px 10px;}
-
<!-- *** End of the alert box *** -->
+
</style>
 +
</html>
 +
<!--- Background Contents --->
 +
<!--- 1st tab --->
 +
<html><br><br><br>
 +
<div id="search-controls">
 +
<form action="/Special:Search" id="searchform">
 +
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" />
 +
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;
 +
      <input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" />
 +
</form>
 +
</div> <!-- close search controls -->
-
{|align="justify"
 
-
|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
 
-
|[[Image:Chiba_logo.png|200px|right|frame]]
 
-
|-
 
-
|
 
-
''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 
-
|[[Image:Your team picture]]
 
-
|-
 
-
|
 
-
|align="center"|[[Team:Chiba | Team Example]]
 
-
|}
 
-
<!--- The Mission, Experiments --->
+
<body bgcolor="blue" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg">
 +
<img src="https://static.igem.org/mediawiki/2010/f/f9/Chiba_menu_2.jpg">
 +
<p>
 +
<style type="text/css">
 +
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
 +
<html xmlns="http://www.w3.org/1999/xhtml">
 +
        <head>
 +
                <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" />
 +
                <title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title>
 +
                <style type="text/css">
 +
<!--
 +
    body {
 +
        margin:0;
 +
        padding:0;
 +
        font: bold 11px/1.5em Verdana;
 +
}
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
h2 {
-
!align="center"|[[Team:Chiba|Home]]
+
        font: bold 14px Verdana, Arial, Helvetica, sans-serif;
-
!align="center"|[[Team:Chiba/Team|Team]]
+
        color: #000;
-
!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Chiba Official Team Profile]
+
        margin: 0px;
-
!align="center"|[[Team:Chiba/Project|Project]]
+
        padding: 0px 0px 0px 15px;
-
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]
+
}
-
!align="center"|[[Team:Chiba/Modeling|Modeling]]
+
-
!align="center"|[[Team:Chiba/Notebook|Notebook]]
+
-
!align="center"|[[Team:Chiba/Safety|Safety]]
+
-
|}
+
 +
img {
 +
border: none;
 +
}
 +
/*- Menu Tabs 10--------------------------- */
 +
    #tabs10 {
 +
      float:left;
 +
      width:100%;
 +
      font-size:100%;
 +
      line-height:normal;
 +
      background:#373737;
 +
      margin-top:-10px;
 +
      }
 +
    #tabs10 ul {
 +
          margin:0;
 +
          padding:2px 10px 0 105px;
 +
          list-style:none;
 +
      }
 +
    #tabs10 li {
 +
      float:left;
 +
      display:inline;
 +
      margin:0;
 +
      padding:0;
 +
      margin-left:40px;
 +
      }
 +
    #tabs10 a {
 +
      float:left;
 +
      background:url("tableft10.gif") no-repeat left top;
 +
      margin:0;
 +
      padding:0 0 0 2px;
 +
      text-decoration:none;
 +
      }
 +
    #tabs10 a span {
 +
      float:left;
 +
      display:block;
 +
      background:url("tabright10.gif") no-repeat right top;
 +
      padding:5px 15px 4px 6px;
 +
      color:#FFF;
 +
      }
 +
    /* Commented Backslash Hack hides rule from IE5-Mac \*/
 +
    #tabs10 a span {float:none;}
 +
    /* End IE5-Mac hack */
 +
    #tabs9 a:hover span {
 +
      color:#FFFFFF;
 +
      }
 +
    #tabs10 a:hover {
 +
      background-position:0% -42px;
 +
      }
 +
    #tabs10 a:hover span {
 +
      background-position:100% -42px;
 +
      }
-
== '''Overall project''' ==
+
      #tabs10 #current a {
 +
              background-position:0% -42px;
 +
      }
 +
      #tabs10 #current a span {
 +
              background-position:100% -42px;
 +
      }
 +
-->
 +
</style>
 +
        </head>
-
<b><font color="blue" size="6">Bacterial counter as the safety device!!</font></b>
+
        <body>
-
<br><br>
+
                <div id="tabs10">
 +
                <ul>
 +
                                <!-- CSS Tabs -->
 +
<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li>
 +
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li>
 +
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li>
 +
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li>
 +
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li>
 +
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li>
 +
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li>
-
We’re inspired by double-click of computer’s mouse. <br>
+
                        </ul>
-
It doesn’t react by first input but react by second input.<br>
+
                </div>
-
So we designed bacterial DNA sequence which works like it.<br>
+
<!--- 1st tab End --->
-
Furthermore, we built time-limit in genetic sequence between the first and the second input like a computer mouse.<br>
+
<!--- 2nd tab --->
-
Bacteria which have the DNA sequence can distinguish between the first input and the second one. <br>
+
<div id="search-controls">
-
Once in a while, bacteria might receive input which wasn’t intended.<br>
+
<form action="/Special:Search" id="searchform">
-
But it was the first input so bacteria don’t react.<br>
+
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" />
-
Even if there was input not intended, bacteria don’t react unless the second input is entered.<br>
+
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;
-
Moreover, when the limit-time which controlled by the genetic system is passed, the system is going to return to the initial state.<br>
+
      <input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" />
-
So we expect this DNA sequence will work as a safety device.<br><br><br><br><br>
+
</form>
 +
</div> <!-- close search controls -->
 +
<p>
 +
<style type="text/css">  
 +
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
 +
<html xmlns="http://www.w3.org/1999/xhtml">
 +
        <head>
 +
                <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" />
 +
                <title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title>
 +
                <style type="text/css">
 +
<!--
 +
    body {
 +
        margin:0;
 +
        padding:0;
 +
        font: bold 11px/1.5em Verdana;
 +
}
-
私たちは、マウスのダブルクリックからアイデアを得て、「1回目の入力ではoutput をせず、2回目の入力ではじめて、output をする」ような遺伝子回路を設計しました。
+
h2 {
-
<br>さらに私たちはマウスと同じく2回目の入力までの時間制限を遺伝子回路に組み込みました。<br>
+
        font: bold 14px Verdana, Arial, Helvetica, sans-serif;
-
大腸菌は、一回目と2回目の入力を見分けられます。<br>
+
        color: #000;
-
もし誤って入力を入れてしまったとしても1回目では、2回目が入らない限り、SWITCHがONとならないので安全装置として働きます。<br>
+
        margin: 0px;
-
時間がたてば1回目の入力はなかったことになります。
+
        padding: 0px 0px 0px 15px;
-
<br>
+
}
-
== Project Details==
+
img {
 +
border: none;
 +
}
 +
/*- Menu Tabs 9--------------------------- */
 +
    #tabs9 {
 +
      float:left;
 +
      width:100%;
 +
      font-size:100%;
 +
      line-height:normal;
 +
      background:#125FDB;
 +
      margin-top:0px;
 +
      }
 +
    #tabs9 ul {
 +
          margin:0;
 +
          padding:2px 10px 0 105px;
 +
          list-style:none;
 +
      }
 +
    #tabs9 li {
 +
      float:left;
 +
      display:inline;
 +
      margin:0;
 +
      padding:0;
 +
      margin-left:40px;
 +
      }
 +
    #tabs9 a {
 +
      float:left;
 +
      background:url("tableft10.gif") no-repeat left top;
 +
      margin:0;
 +
      padding:0 0 0 2px;
 +
      text-decoration:none;
 +
      }
 +
    #tabs9 a span {
 +
      float:left;
 +
      display:block;
 +
      background:url("tabright10.gif") no-repeat right top;
 +
      padding:5px 15px 4px 6px;
 +
      color:#FFF;
 +
      }
 +
    /* Commented Backslash Hack hides rule from IE5-Mac \*/
 +
    #tabs9 a span {float:none;}
 +
    /* End IE5-Mac hack */
 +
    #tabs9 a:hover span {
 +
      color:#FFFFFF;
 +
      }
 +
    #tabs9 a:hover {
 +
      background-position:0% -42px;
 +
      }
 +
    #tabs9 a:hover span {
 +
      background-position:100% -42px;
 +
      }
 +
      #tabs9 #current a {
 +
              background-position:0% -42px;
 +
      }
 +
      #tabs9 #current a span {
 +
              background-position:100% -42px;
 +
      }
 +
-->
 +
</style>
 +
        </head>
 +
        <body>
 +
                <div id="tabs9">
 +
                <ul>
 +
                                <!-- CSS Tabs -->
 +
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Double Click System</span></a></li>
 +
<li><a href="https://2010.igem.org/Team:Chiba/System_1"><span>Version 1</span></a></li>
 +
<li><a href="https://2010.igem.org/Team:Chiba/System_2"><span>Version 2</span></a></li>                      </ul>
 +
                </div>
 +
        </body>
 +
</html>
-
=== Part 2 ===
+
<!--- 2nd tab End--->
 +
<!--- Background Contents End --->
 +
<!-- -------------------------------------------------------------------------------
 +
-------------------------------------------------------------------------------
 +
ここから下を編集
 +
-------------------------------------------------------------------------------
 +
----------------------------------------------------------------------------------- -->
 +
<br><br><br>
 +
===<font size="5">Double Click Bacteria</font>===
 +
-----
 +
We’re inspired by double-click of computer’s mouse. It doesn’t react to the first click but does react when it is accompanied by the second one. This is one of the most accepted, familiarized, and proven mechanism to diminish the erroneous operation. This fail-safe technology should find various uses also in biotechnology.
 +
Here is what we envision: once in a while, bacteria might receive input which wasn’t intended. But they are careful enough (they know Homo sapiens always make mistake!) so that they ignore the input for the first time.  However, when they receive the two inputs in succession, they finally judge the input(s) are real, and start taking the action as programmed.  Also, they are smart enough to distinguish double-clicking from the two separate (or erroneous) inputs.
 +
First, the circuit must distinguish the second input (stimulus) from the first one, even though they are chemically physically the same . To realize this, we need the counting device. Second, the circuit must distinguishes W-click from two separate clicks; the circuit reacts only when the first and the second input are given in short period of time. Thus, the genetic counter should be coupled with the genetic timers. After a certain time after the first input (timeout), the circuit gets back to the initial state so that the  next stimulous is recognized as the first input.
 +
<html>
 +
<center>
 +
<table width="80%"><tr><td>
 +
<img src="https://static.igem.org/mediawiki/2010/7/7c/Click.jpg"></td><td>
 +
<object width="480" height="385"><param name="movie" value="http://www.youtube.com/v/vQbNnGbuXj0?fs=1&amp;hl=ko_KR"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="http://www.youtube.com/v/vQbNnGbuXj0?fs=1&amp;hl=ko_KR" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="480" height="385"></embed></object>
 +
</td></tr></table>
 +
</center>
 +
</html>
 +
<br><br><br>
 +
===<font size="5">The Bottom Line is...</font>===
 +
#If there is only an input, nothing happens.
 +
#Duration of the input is not the matter. The circuit cares only the number of input.
 +
#However, a certain time after the 1st input, it returns to the initial state.
 +
#Giving two inputs in the limited time the circuit get activated (gives output).
 +
<Br><br>
-
=== The Experiments ===
+
===<font size="5">Systems</font>===
-
 
+
<html>
-
 
+
<center>
-
 
+
<table width="80%" border="0"><tr><td style="text-align:center;">
-
 
+
<a href="https://2010.igem.org/Team:Chiba/System_1"><img src="https://static.igem.org/mediawiki/2010/d/db/Chiba_plan2_1.jpg" border="0" width="300px" /><br>version 1</a>
-
=== Part 3 ===
+
</td><td style="text-align:center;">
-
 
+
<a href="https://2010.igem.org/Team:Chiba/System_2"><img src="https://static.igem.org/mediawiki/2010/1/1a/Chiba_Sys2.jpg" border="0" width="300px" /><br>version 2</a>
-
 
+
</td></tr></table>
-
 
+
</center>
-
 
+
</html>
-
== Results ==
+

Latest revision as of 03:10, 28 October 2010




 

 




Double Click Bacteria

We’re inspired by double-click of computer’s mouse. It doesn’t react to the first click but does react when it is accompanied by the second one. This is one of the most accepted, familiarized, and proven mechanism to diminish the erroneous operation. This fail-safe technology should find various uses also in biotechnology.

Here is what we envision: once in a while, bacteria might receive input which wasn’t intended. But they are careful enough (they know Homo sapiens always make mistake!) so that they ignore the input for the first time. However, when they receive the two inputs in succession, they finally judge the input(s) are real, and start taking the action as programmed. Also, they are smart enough to distinguish double-clicking from the two separate (or erroneous) inputs.

First, the circuit must distinguish the second input (stimulus) from the first one, even though they are chemically physically the same . To realize this, we need the counting device. Second, the circuit must distinguishes W-click from two separate clicks; the circuit reacts only when the first and the second input are given in short period of time. Thus, the genetic counter should be coupled with the genetic timers. After a certain time after the first input (timeout), the circuit gets back to the initial state so that the next stimulous is recognized as the first input.




The Bottom Line is...

  1. If there is only an input, nothing happens.
  2. Duration of the input is not the matter. The circuit cares only the number of input.
  3. However, a certain time after the 1st input, it returns to the initial state.
  4. Giving two inputs in the limited time the circuit get activated (gives output).



Systems


version 1
version 2

Retrieved from "http://2010.igem.org/Team:Chiba/Project"