Team:Chiba/Project

From 2010.igem.org

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<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Overall project</span></a></li>
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<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Double Click System</span></a></li>
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<li><a href="https://2010.igem.org/Team:Chiba/System_1"><span>System 1</span></a></li>
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<li><a href="https://2010.igem.org/Team:Chiba/System_1"><span>Version 1</span></a></li>
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<li><a href="https://2010.igem.org/Team:Chiba/System_2"><span>System 2</span></a></li>                       </ul>
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<li><a href="https://2010.igem.org/Team:Chiba/System_2"><span>Version 2</span></a></li>                       </ul>
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===<font size="5">Double Click Bacteria</font>===
===<font size="5">Double Click Bacteria</font>===
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If there are two-time input in fixed time, bacteria is going to shine.
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We’re inspired by double-click of computer’s mouse. It doesn’t react to the first click but does react when it is accompanied by the second one. This is one of the most accepted, familiarized, and proven mechanism to diminish the erroneous operation. This fail-safe technology should find various uses also in biotechnology.  
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<br><br><br>
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===<font size="5">Overall Project</font>===
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Here is what we envision: once in a while, bacteria might receive input which wasn’t intended. But they are careful enough (they know Homo sapiens always make mistake!) so that they ignore the input for the first time. However, when they receive the two inputs in succession, they finally judge the input(s) are real, and start taking the action as programmed.  Also, they are smart enough to distinguish double-clicking from the two separate (or erroneous) inputs.
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First, the circuit must distinguish the second input (stimulus) from the first one, even though they are chemically physically the same . To realize this, we need the counting device. Second, the circuit must distinguishes W-click from two separate clicks; the circuit reacts only when the first and the second input are given in short period of time. Thus, the genetic counter should be coupled with the genetic timers. After a certain time after the first input (timeout), the circuit gets back to the initial state so that the  next stimulous is recognized as the first input.
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[[Image:Click.jpg]]
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We’re inspired by double-click of computer’s mouse.
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<img src="https://static.igem.org/mediawiki/2010/7/7c/Click.jpg"></td><td>
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It doesn’t react by first input but react by second input. So we designed bacterial DNA sequence which works like it. Furthermore, we built time-limit in genetic sequence between the first and the second input like a computer mouse. Bacteria which have the DNA sequence can distinguish between the first input and the second one. Once in a while, bacteria might receive input which wasn’t intended. But it was the first input so bacteria don’t react. Even if there was input not intended, bacteria don’t react unless the second input is entered. Moreover, when the limit-time which controlled by the genetic system is passed, the system is going to return to the initial state. So we expect this DNA sequence will work as a safety device.
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<object width="480" height="385"><param name="movie" value="http://www.youtube.com/v/vQbNnGbuXj0?fs=1&amp;hl=ko_KR"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="http://www.youtube.com/v/vQbNnGbuXj0?fs=1&amp;hl=ko_KR" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="480" height="385"></embed></object>
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<br><br><br>
<br><br><br>
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===<font size="5">Requirement of Double Click</font>===
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===<font size="5">The Bottom Line is...</font>===
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----
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#If there is only an input, nothing happens.
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#If there is a click only one-time, nothing happens.
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#Duration of the input is not the matter. The circuit cares only the number of input.
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#Time of clicking button doesn't matter. It just depends on the number of click. One or two.
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#However, a certain time after the 1st input, it returns to the initial state.
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#After a lot of time pass from the 1st click, it will return to the initial state.
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#Giving two inputs in the limited time the circuit get activated (gives output).
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#Click two-time in specific limited time(We call this double click.), something occur.
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<Br><br>
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===<font size="5">Systems</font>===
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<center>
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<table width="80%" border="0"><tr><td style="text-align:center;">
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<a href="https://2010.igem.org/Team:Chiba/System_1"><img src="https://static.igem.org/mediawiki/2010/d/db/Chiba_plan2_1.jpg" border="0" width="300px" /><br>version 1</a>
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</td><td style="text-align:center;">
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<a href="https://2010.igem.org/Team:Chiba/System_2"><img src="https://static.igem.org/mediawiki/2010/1/1a/Chiba_Sys2.jpg" border="0" width="300px" /><br>version 2</a>
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Latest revision as of 03:10, 28 October 2010




 

 




Double Click Bacteria

We’re inspired by double-click of computer’s mouse. It doesn’t react to the first click but does react when it is accompanied by the second one. This is one of the most accepted, familiarized, and proven mechanism to diminish the erroneous operation. This fail-safe technology should find various uses also in biotechnology.

Here is what we envision: once in a while, bacteria might receive input which wasn’t intended. But they are careful enough (they know Homo sapiens always make mistake!) so that they ignore the input for the first time. However, when they receive the two inputs in succession, they finally judge the input(s) are real, and start taking the action as programmed. Also, they are smart enough to distinguish double-clicking from the two separate (or erroneous) inputs.

First, the circuit must distinguish the second input (stimulus) from the first one, even though they are chemically physically the same . To realize this, we need the counting device. Second, the circuit must distinguishes W-click from two separate clicks; the circuit reacts only when the first and the second input are given in short period of time. Thus, the genetic counter should be coupled with the genetic timers. After a certain time after the first input (timeout), the circuit gets back to the initial state so that the next stimulous is recognized as the first input.




The Bottom Line is...

  1. If there is only an input, nothing happens.
  2. Duration of the input is not the matter. The circuit cares only the number of input.
  3. However, a certain time after the 1st input, it returns to the initial state.
  4. Giving two inputs in the limited time the circuit get activated (gives output).



Systems


version 1
version 2

Retrieved from "http://2010.igem.org/Team:Chiba/Project"