Team:Hong Kong-CUHK

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==Project Background==
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With the advancement of electronic engineering after the World War II, more complex ciphers are developed, which plays a pivotal role in the security system of this information explosive age. However mathematical advances result in the weakening or even attack on the ciphers. It makes us rethink whether data storage and encryption in computer is the only way to ensure data safety.
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Using bacteria as the information storage device is not a new idea, Bancroft’s group [1] had long proposed the storage of information in DNA early in 2001. Yachie’s group[2] had also been working on the bacterial data storage method in ''Bacillus subtilis'' in 2007. In contrast to electronic data storage, the nature of bacterial data storage depends on the bacteria one would pick – ''Bacillus subtilis'' would create extra copies of the data, inserting into their genomes which would further safeguard the information; ''Deinococcus radiodurans'', one of the most radioresistant organisms known, would survive even under the electromagnetic pulse and radiation after the nuclear attack. But how are we actually improving the security system by simply storing information in bacteria?
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==Not re-inventing the wheel==
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This year in the iGEM 2010 competition, we will use bacteria not only as a biological data storage unit but also to integrate an intrinsic encryption system with it.
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Our data storage system is novel. We focus on using multiple bacteria as a parallel data storage model, for which involves the fragmentation of original information into tiny pieces and the incorporation of the fragmented information into the bacteria, which varies greatly from using single bacterial cell as the information storage unit as demonstrated by the previous group. Together with the encryption system, the security of the data is further ensured.
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To retrieve the data, the information DNA would be sequenced after isolation from the bacteria. Computational algorithms are designed to resolve the encryption system and the original information could then be recovered.
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==Expectation/goal==
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===Ultimate storage capacity===
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Our expectation is high. A droplet of liquid can contain more than a billion bacteria – the potential capacity of this bacterial information storage unit is tremendous.
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CUHK IGEM 2010--Bioencryption</h1>
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===Setup industrial standard on bacterial information storage===
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To generalize the bacterial storage system, we will setup the industrial standard of DNA information storage on a parallel basis – multiple bacteria approach, for which is still applicable to single cell storage.
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===Future security protocol===
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To take advantage of the nature of the bacteria as well as the intrinsic data encryption system in the bacterial information storage unit, this would be a new way to securely store data forever.
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Our Project </h2>
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<p><span style="font-family: Optima, Lucida, 'MgOpen Cosmetica', 'Lucida Sans Unicode', sans-serif; line-height: 17px; font-size: 13px;"><p><span class="dropcap-orange">D</span></span>ata encryption and storage has always been an important branch of research in computer engineering. In our project, we explored the possibility of harnessing a biological system as an alternative solution for data en/decryption and storage. Using bacteria as the information storage device is not new.<span> </span>However the practicability of previous research is being doubt due to the limited size of information available to be inserted into the bacteria.</p></p>
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<p> </p>
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<p><span style="font-family: Optima, Lucida, 'MgOpen Cosmetica', 'Lucida Sans Unicode', sans-serif; line-height: 17px; font-size: 13px;"><p><span class="dropcap-purple">W</span></span>e recognized the current barricades in developing a truly useful system and we forecasted the indispensable modules that one would be anticipating when putting fantasy into reality. This year, we have proposed a model that is a true, <span class="highlight-purple">massively parallel bacterial data storage</span> system.</p></p>
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<p> </p>
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<p><span style="font-family: Optima, Lucida, 'MgOpen Cosmetica', 'Lucida Sans Unicode', sans-serif; line-height: 17px; font-size: 13px;"><p><span class="dropcap-brown">I</span></span>n addition we have created an <span class="highlight-purple">encryption module</span> with the R64 Shufflon-Specific Recombinase to further secure the information. Together with the data proof-read/correction and random access modules developed, our expectation is high - we believe this could be an industrial standard in handling large scale data storage in living cells.</p></p>
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<p> </p>
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<span class="leading_separator">&nbsp;</span>
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==References==
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[1] Carter Bancroft et al, Long-Term Storage of Information in DNA, Science
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<p>Since March 2010, our team has been intensively working on this project. Learn more about our team members by visiting the team page.</p>
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<p style="text-align: right;"><a class="readon" href="https://2010.igem.org/Team:Hong_Kong-CUHK/Team"><span>Read more</span></a></p>
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<p>To better illustrate our project, we have created simulation software and modeled the bioencryption platform <em>in silico.</em> Click to read more.</p>
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<p style="text-align: right;"><a class="readon" href="https://2010.igem.org/Team:Hong_Kong-CUHK/Model"><span>Read more</span></a></p>
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<p>The Chinese University of Hong Kong IGEM team would like to thank our generous sponsors. Click the read more link for more information.</p>
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<p style="text-align: right;"><a class="readon" href="https://2010.igem.org/Team:Hong_Kong-CUHK/Sponsors"><span>Read more</span></a></p>
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<p>We enjoyed so much working on our project, and we had good time with other iGEM members in 2010 spring workshop.</p>
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<p style="text-align: right;"><a class="readon" href="https://2010.igem.org/Team:Hong_Kong-CUHK/Team_gallery"><span>Read more</span></a></p>
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<table border="0" width="250">
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<tbody>
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<td height="150">
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<p>We have created and submitted new biobricks, which can help keep your message SECRET and SAFE from prying eyes ! Click to read more.</p>
 +
<p style="text-align: right;"><a class="readon" href="https://2010.igem.org/Team:Hong_Kong-CUHK/Parts"><span>Read more</span></a></p>
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<td height="150">
 +
<p>Know more about our work progress by browsing our NOTEBOOK! There are also lots of useful protocols for bacterial transformation.</p>
 +
<p style="text-align: right;"><a class="readon" href="https://2010.igem.org/Team:Hong_Kong-CUHK/Notebook"><span>Read more</span></a></p>
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<h3 class="module-title">Project highlights</h3>
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<p><ul class="special-7"><li>Massively parallel bacterial data storage system</li><li>Error tolerant data encoding/decoding system</li><li>Recombination module for data encryption</li><li>Ready for the exciting future of Biocomputer!</li></ul></p> </div>
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<h3 class="module-title">Countdown to iGEM</h3>
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[2] Nozomu Yachie et al, Alignment-Based Approach for Durable Data Storage into Living Organisms, Biotechnology Progress. 2007, 23, 501−505
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|[[Image:Hong_Kong-CUHK_team.png|right|frame|Your team picture]]
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<h3 class="module-title">Quick Links</h3>
 +
<p><strong>The Chinese University of Hong Kong<br /></strong><a href="http://www.cuhk.edu.hk/">CUHK</a></br> - <a href="http://www.bch.cuhk.edu.hk/">Dept. of Biochemistry</a></br> - <a href="http://www.cuhk.edu.hk/sci/lifesciences.htm">Sch. of Life Sciences</a> <br /><br /><strong>IGEM</strong><br /><a href="https://igem.org/Main_Page">IGEM Main Page</a></br> - <a href="http://partsregistry.org/Main_Page">Registry of Standard Biological Parts</a><br /> - <a href="https://igem.org/Judging_Form.cgi?id=361">Judging form</a><br /></p> </div>
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 +
<p>The International Genetically Engineered Machine competition (iGEM) is the premiere undergraduate Synthetic Biology competition.</p>
 +
<p style="text-align: right;"><a class="readon" href="https://igem.org/Main_Page"><span>Read more</span></a></p> </div>
 +
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Latest revision as of 02:51, 28 October 2010

CUHK IGEM 2010--Bioencryption

CUHK IGEM 2010--Bioencryption

Our Project

Data encryption and storage has always been an important branch of research in computer engineering. In our project, we explored the possibility of harnessing a biological system as an alternative solution for data en/decryption and storage. Using bacteria as the information storage device is not new. However the practicability of previous research is being doubt due to the limited size of information available to be inserted into the bacteria.

 

We recognized the current barricades in developing a truly useful system and we forecasted the indispensable modules that one would be anticipating when putting fantasy into reality. This year, we have proposed a model that is a true, massively parallel bacterial data storage system.

 

In addition we have created an encryption module with the R64 Shufflon-Specific Recombinase to further secure the information. Together with the data proof-read/correction and random access modules developed, our expectation is high - we believe this could be an industrial standard in handling large scale data storage in living cells.

 

 

Since March 2010, our team has been intensively working on this project. Learn more about our team members by visiting the team page.

Read more

To better illustrate our project, we have created simulation software and modeled the bioencryption platform in silico. Click to read more.

Read more

The Chinese University of Hong Kong IGEM team would like to thank our generous sponsors. Click the read more link for more information.

Read more

We enjoyed so much working on our project, and we had good time with other iGEM members in 2010 spring workshop.

Read more

We have created and submitted new biobricks, which can help keep your message SECRET and SAFE from prying eyes ! Click to read more.

Read more

Know more about our work progress by browsing our NOTEBOOK! There are also lots of useful protocols for bacterial transformation.

Read more

Project highlights

  • Massively parallel bacterial data storage system
  • Error tolerant data encoding/decoding system
  • Recombination module for data encryption
  • Ready for the exciting future of Biocomputer!

Countdown to iGEM

iGEM 05.11.2010 00:00 7 Days

Quick Links

The Chinese University of Hong Kong
CUHK
- Dept. of Biochemistry
- Sch. of Life Sciences

IGEM
IGEM Main Page
- Registry of Standard Biological Parts
- Judging form

What's IGEM?

The International Genetically Engineered Machine competition (iGEM) is the premiere undergraduate Synthetic Biology competition.

Read more

Retrieved from "http://2010.igem.org/Team:Hong_Kong-CUHK"