Team:Tec-Monterrey/Headquarters

From 2010.igem.org

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<ul>
<ul>
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li>
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li>
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<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors</a></li>
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<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li>
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<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li>
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li>
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li>
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<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p>
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<a href="#">&nbsp;&nbsp;Project</a></p>
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<ul>
<ul>
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<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">Project Overview</a></li>
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<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li>
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                         <li><a href="https://2010.igem.org/Team:Tec-Monterrey/Biosensor">The Biosensor</a></li>
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                         <li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li>
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<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Biosensor">Applications</a></li>
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<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li>
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<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li>
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<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p>
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<a href="#">&nbsp;&nbsp;Notebook</a></p>
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                         <li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li>
                         <li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li>
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        <li><a href="https://2010.igem.org/Team:Tec-Monterrey/Timeline">Timeline</a></li>
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        <li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li>
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                         <li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li>
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</li>
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<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p>
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                        <li><a href="https://2010.igem.org/Team:Tec-Monterrey/News">News</a></li>
 
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li>
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li>
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                        <li><a href="https://2010.igem.org/Team:Tec-Monterrey/Freetime">Free Time</a></li>
 
                         <li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li>
                         <li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li>
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<h1>Project Summary</h1>
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<a href="#main"><li>Main Headquarters
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<a href="#femsa"><li> FEMSA Biotechnology Center </a></li>
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<a href="#ouruniversity"><li> Our University </a></li>
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<a name="main"></a> <h2> Main Headquarters </h2>
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<img src="https://static.igem.org/mediawiki/2010/2/23/Cb.jpg" alt="Biotechnology Center">
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Our headquarters for the development of this project were the facilities at the FEMSA Biotechnology Center at the Tecnológico de Monterrey in Monterrey, Mexico. At this center, we were kindly given access to the <b>Laboratory of Genetic Manipulation and Molecular Diagnostics</b>, which became our main workplace throughout the summer. It was in this laboratory that we had all the necessary equipment to perform most of our experiments: DNA extractions, gel electrophoresis, ligations, digestions and gel purifications, as well as space to carry out research for the development of the project.
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</p>  
<p class="blah">
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      Bacterial reporters or whole-cell bacterial sensors have always been an area of application for genetic manipulation and synthetic biology. As a matter of fact, constructing a bioreporter bacteria that has the ability to detect toxic chemicals is considered one of the first accomplishments in the discipline of synthetic biology <a href="#van">(van der Meer and Belkin, 2010)</a>. There are several advantages to using a bacterial bioreporter instead of a traditional physical or chemical sensors, for example, bacteria can offer the same specificity and sensitivity that traditional sensors offer, but they are much more portable and grow in inexpensive media. Furthermore, bacterial reporters also offer advantages over using other types of biosensors like enzymes and antibodies because they are living organisms and they are capable of analyzing samples through a process that involves many enzymes <a href="#yagi">(Yagi, 2007)</a>
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We also had access to the <b>Fifth Floor Research Facilities at the Biotechnology Center</b>, where we performed the part of the project that demanded much more sophisticated equipment, such as transformations by electroporation, quantifications and fluorescence assays.
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      The first bacterial reporters appeared 20 years ago <a href="van">(van der Meer and Belkin, 2010)</a> although these early tests didn’t use genetically modified microorganisms. Further research and development in the areas of genetic engineering and synthetic biology have resulted in many more applications such as detection of contaminants <a href="#willardson">(Willardson, et. Al., 1998)</a> and sugar and amino acid availability in soils <a href="#jaeger">(Jaeger, et. Al., 1999)</a>. Even though the reporters have gotten more sophisticated and sensitive, we realized that there isn’t much mention of a single bacterial bioreporter capable of detecting different concentrations of a substance and reacting differently depending on the concentration.
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      We thought the use of synthetic biology as well as the BioBrick standard could help create a “genetic circuit” <a href="#van">(van der Meer and Belkin, 2010)</a> capable of detecting different concentrations of a substance and reacting in a different manner depending on the amount detected. In our initial research we realized that the iGEM British Columbia 2009 team decided to do something similar, so we used parts of their project as a base and integrated parts of other previous iGEM projects in order to propose a new genetic construction capable of detecting different amounts of a certain substance. We call these new types of sensors, “intelligent biosensors”, because they have the ability to react in different ways depending on their surroundings.
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      With our project we hope to continue with the previous efforts of other iGEM teams, and at the same time propose a new type of genetic circuit for achieving these functions. In the process we plan to develop BioBricks for two new families of phage activators as well as different BioBrick constructons that can make our system easy to adapt, so that the creation of these “intelligent biosensors” becomes just a matter of choosing the substance of interest and choosing the different reporters.
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Additionaly, part of our work had to be done at the <b>ITESM School of Medicine</b>, namely the preparation of electrocompetent cells.
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<a name="femsa"></a> <h2> FEMSA Biotechnology Center </h2>
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<img src="https://static.igem.org/mediawiki/2010/8/89/Cbatras.jpg" alt="Biotechnology Center">
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This Center, located at the Tecnológico de Monterrey in Monterrey, Mexico, is devoted to the generation of research, the transfer of knowledge and the modeling of new business schemes that involve biotechnology and food technology areas. For such reasons, it is equiped with cutting-edge infraestructure in laboratories, pilot plants and business incubators.
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</p>
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<p class="blahimgder">
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The Center emerged 15 years ago as a response to the increasing trends in scientific and technologic innovation in the country and the whole world and through time it has supported the following lines of research, all focused in the innovation of products, processes and services:
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<ol>
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<li><b>Food Biotechnology:</b></li>
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<ul><li>Study of biomolecules found in Mexican plants and foods with therapeutic properties. </li>
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<li>Development and optimization of new food products and processes. </li>
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</ul>
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<li><b>Bioprocess Engineering:</b></li>
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<ul><li>Design, scaling and optimization of biotechnological processes. </li>
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</ul>
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<li><b>Pharmaceutical and Medical Biotechnology:</b> </li>
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<ul><li>Development and validation of new drugs. </li>
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<li>Molecular diagnostic of diseases. </li>
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<li>Culture and differentiation of tissues. </li>
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</ul>
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<li><b>Biological Chemistry:</b> </li>
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<ul><li>Isolation and characterization of biologically active compounds. </li>
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<li>Synthesis of new drugs</li>
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<li>Design of new materials with applications in the pharmaceutical and biotechnological industries. </li></ul>
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</ol>
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</p>
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<p class="blah">
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<b>Some of the strategic partners of the center are:</b>
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<ul>
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<li>Cambridge University, UK</li>
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<li>Texas A&M University</li>
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<li>Cornell University</li>
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<li>Arizona State University</li>
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<li>University of California (San Diego & Davis) </li>
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<li>Carnegie Mellon University</li>
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</ul>
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</p>
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<p class="blah">
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<b>Some of the achievements of the center are:</b>
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<ul>
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<li>More than 14 patent requests.</li>
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<li>More than 250 papers published in journals with high indexing level.</li>
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<li>3 published books.</li>
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<li>12 book chapters</li>
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<li>30 researches, of which, more than 70% belong to the National System of Researchers.
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</ul>
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</p>
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<center>
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<p class="imgcenter">
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<img src="https://static.igem.org/mediawiki/2010/8/80/Cbflores.jpg">
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</p>
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</center>
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<a name="ouruniversity"></a> <h2> Our University </h2>
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<p class="blahimgizq">
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<img src="https://static.igem.org/mediawiki/igem.org/b/b9/Itesm1.jpg" alt="ITESM">
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The Monterrey Institute of Higher Education, or in Spanish Instituto Tecnológico y de Estudios Superiores de Monterrey (ITESM, or simply Tec de Monterrey) is one of the largest private, non-profit, independent coeducational multi-campus universities with no political or religious affiliation in Latin America. It is based in Monterrey, Mexico and has 33 campuses throughout the country, in addition to international presence through liaison offices in 12 foreign countries. It has one of the top graduate business schools in the region (EGADE) and is one of the leaders in patent applications among Mexican universities.
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</p>
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<p class="blahimgder">
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<img src="https://static.igem.org/mediawiki/igem.org/a/a0/Itesm2.jpg" alt="ITESM">
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The Tecnológico de Monterrey was founded in 1943 as a result of the far-reaching vision and the commitment of Don Eugenio Garza Sada and a group of businessmen. Today, when reality has outreached the original concept, the Tecnológico de Monterrey includes practically all the areas of knowledge, having an educational offering that consists of a wide assortment of undergraduate majors, specialties, Masters Degree programs, medical specialty programs, and Ph.D. programs in the fields of Agriculture, Architecture, Biotechnology, Business and Management, Communications, Design and Applied Art, Engineering and Sciences, Health, Humanities and Social Sciences, Information Technology, and Law, among others.
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</p>
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<p class="blahimgizq">
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<img src="https://static.igem.org/mediawiki/igem.org/0/04/Itesm3.jpg" alt="ITESM">
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The Tecnológico de Monterrey is working to be the most widely recognized educational institution in Latin America for the leadership exercised by alumni in the private, public, and social sectors, and for the research and technological development it carries out to promote a knowledge-based economy, generating management and business incubators models, improving public administration and public policies, and creating innovative models and systems for the sustainable development of the community.
 +
</p>
 +
<p class="blahimgder">
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The educational model emphasizes collaborative work and uses didactic techniques such as problem-based learning, project-oriented learning, and the case-solving method. In this way knowledge is applied to solving real-life problems, it makes studying meaningful and becomes the object of critical reflection and social commitment. There is also a big emphasis and commitment on generating knowledge and creating an entrepreneurial culture.
 +
</p>
 +
<p class="blahimgder">
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During the past years, the Institution has turned its attention to biotechnology, heavily investing in infrastructure, teaching and laboratory equipment. This includes the FEMSA Biotechnology Center, which is an intellectual hub for the different researchers and students that come together. The Institute now offers undergraduate, M.Sc. and Ph.D. studies in Biotechnology in different fields such as Food Biotechnology, Bioprocess Engineering, Biopharmaceuticals and Bioproducts.
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</p>
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<p class="blahimgder">
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For more information, we encourage you to visit our university's <a href="http://www.itesm.edu/wps/portal/english?WCM_GLOBAL_CONTEXT">website</a>.
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</p>
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<center>
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<img src="https://static.igem.org/mediawiki/igem.org/b/b7/Itesm4.jpg">
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</center>
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</div>
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<h2>References</h2>
 
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<p class="blah"><a name="jaeger">Jaeger, C. H., et. Al. (June 1999)</a> Mapping of Sugar and Amino Acid Availability in Soil around Roots with Bacterial Sensors of Sucrose and Tryptophan. Applied and Environmental Microbiology, Vol. 65, No. 6, p. 2685 - 2690
 
-
<br><br>
 
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<a name="van">Van der Meer, J. R. and Belkin, S. (July 2010)</a> Where microbiology meets microengineering: design and applications of reporter bacteria. Nature Reviews Microbiology, Vol. 8, p. 511 - 522
 
-
<br>
 
-
<br>
 
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<a name="willardson">Willardson, B. M., et. Al. (March 1998)</a> Development and Testing of a Bacterial Biosensor for Toluene-Based Environmental Contaminants. Applied and Environmental Microbiology, Vol. 64, No. 3, p. 1006- 1012
 
-
<br><br>
 
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<a name="yagi">Yagi, K. (2007)</a> Applications of whole-cell bacterial sensors in biotechnology and environmental science. Applied Microbiology and Biotechnology, Vol. 73, p. 1251 - 1258
 
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<br><br></p>
 
</div>
</div>
<div class="ft"><div class="c"></div></div>
<div class="ft"><div class="c"></div></div>

Latest revision as of 00:56, 28 October 2010

Tec de Monterrey





























Main Headquarters

Biotechnology Center Our headquarters for the development of this project were the facilities at the FEMSA Biotechnology Center at the Tecnológico de Monterrey in Monterrey, Mexico. At this center, we were kindly given access to the Laboratory of Genetic Manipulation and Molecular Diagnostics, which became our main workplace throughout the summer. It was in this laboratory that we had all the necessary equipment to perform most of our experiments: DNA extractions, gel electrophoresis, ligations, digestions and gel purifications, as well as space to carry out research for the development of the project.

We also had access to the Fifth Floor Research Facilities at the Biotechnology Center, where we performed the part of the project that demanded much more sophisticated equipment, such as transformations by electroporation, quantifications and fluorescence assays.

Additionaly, part of our work had to be done at the ITESM School of Medicine, namely the preparation of electrocompetent cells.

FEMSA Biotechnology Center

Biotechnology Center This Center, located at the Tecnológico de Monterrey in Monterrey, Mexico, is devoted to the generation of research, the transfer of knowledge and the modeling of new business schemes that involve biotechnology and food technology areas. For such reasons, it is equiped with cutting-edge infraestructure in laboratories, pilot plants and business incubators.

The Center emerged 15 years ago as a response to the increasing trends in scientific and technologic innovation in the country and the whole world and through time it has supported the following lines of research, all focused in the innovation of products, processes and services:

  1. Food Biotechnology:
    • Study of biomolecules found in Mexican plants and foods with therapeutic properties.
    • Development and optimization of new food products and processes.
  2. Bioprocess Engineering:
    • Design, scaling and optimization of biotechnological processes.
  3. Pharmaceutical and Medical Biotechnology:
    • Development and validation of new drugs.
    • Molecular diagnostic of diseases.
    • Culture and differentiation of tissues.
  4. Biological Chemistry:
    • Isolation and characterization of biologically active compounds.
    • Synthesis of new drugs
    • Design of new materials with applications in the pharmaceutical and biotechnological industries.

Some of the strategic partners of the center are:

  • Cambridge University, UK
  • Texas A&M University
  • Cornell University
  • Arizona State University
  • University of California (San Diego & Davis)
  • Carnegie Mellon University

Some of the achievements of the center are:

  • More than 14 patent requests.
  • More than 250 papers published in journals with high indexing level.
  • 3 published books.
  • 12 book chapters
  • 30 researches, of which, more than 70% belong to the National System of Researchers.

Our University

ITESM The Monterrey Institute of Higher Education, or in Spanish Instituto Tecnológico y de Estudios Superiores de Monterrey (ITESM, or simply Tec de Monterrey) is one of the largest private, non-profit, independent coeducational multi-campus universities with no political or religious affiliation in Latin America. It is based in Monterrey, Mexico and has 33 campuses throughout the country, in addition to international presence through liaison offices in 12 foreign countries. It has one of the top graduate business schools in the region (EGADE) and is one of the leaders in patent applications among Mexican universities.

ITESM The Tecnológico de Monterrey was founded in 1943 as a result of the far-reaching vision and the commitment of Don Eugenio Garza Sada and a group of businessmen. Today, when reality has outreached the original concept, the Tecnológico de Monterrey includes practically all the areas of knowledge, having an educational offering that consists of a wide assortment of undergraduate majors, specialties, Masters Degree programs, medical specialty programs, and Ph.D. programs in the fields of Agriculture, Architecture, Biotechnology, Business and Management, Communications, Design and Applied Art, Engineering and Sciences, Health, Humanities and Social Sciences, Information Technology, and Law, among others.

ITESM The Tecnológico de Monterrey is working to be the most widely recognized educational institution in Latin America for the leadership exercised by alumni in the private, public, and social sectors, and for the research and technological development it carries out to promote a knowledge-based economy, generating management and business incubators models, improving public administration and public policies, and creating innovative models and systems for the sustainable development of the community.

The educational model emphasizes collaborative work and uses didactic techniques such as problem-based learning, project-oriented learning, and the case-solving method. In this way knowledge is applied to solving real-life problems, it makes studying meaningful and becomes the object of critical reflection and social commitment. There is also a big emphasis and commitment on generating knowledge and creating an entrepreneurial culture.

During the past years, the Institution has turned its attention to biotechnology, heavily investing in infrastructure, teaching and laboratory equipment. This includes the FEMSA Biotechnology Center, which is an intellectual hub for the different researchers and students that come together. The Institute now offers undergraduate, M.Sc. and Ph.D. studies in Biotechnology in different fields such as Food Biotechnology, Bioprocess Engineering, Biopharmaceuticals and Bioproducts.

For more information, we encourage you to visit our university's website.