Team:Peking

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<p><br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<font size=4>Biosensor:</font><br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Based on reverse engineering principles, we<br>
 
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&nbsp;&nbsp;&nbsp;engineered our bacteria into valid bioreporters <br>&nbsp;&nbsp;&nbsp;for Hg(II) in aquatic environment. Rational desi-<br>&nbsp;&nbsp;&nbsp;-gn of genetic circuit topology was conducted to <br>&nbsp;&nbsp;&nbsp;confer mercury sensor and regulator compone-<br>&nbsp;&nbsp;&nbsp;-nts high efficiency and robustness. Additionally,<br>&nbsp;&nbsp;&nbsp; a new approach based on the use of multiple <br>&nbsp;&nbsp;&nbsp;bioreporter cell lines to control assay variations <br>&nbsp;&nbsp;&nbsp;and to improve in field application ease was also <br>&nbsp;&nbsp;&nbsp;developed. <br>
 
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://2010.igem.org/Team:Peking/Project/Biosensor"><font size=4>---learn more</font></a></p>
 
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<p><br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<font size=4>Expansion:</font>
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&nbsp;&nbsp;&nbsp;We noticed that MerR family TFs share a high ho-<br>&nbsp;&nbsp;&nbsp;-mology at their metal binding domains, which implies that our strategies of &nbsp;&nbsp;&nbsp;bioreporter and bioabsorbent engineering might be applicable to other cases. <br>&nbsp;&nbsp;&nbsp;Then our reverse engineering strategy was expanded to another common to-<br>&nbsp;&nbsp;&nbsp;-xic heavy metal, lead (Pb) to prove the validness of our engineering strategy.
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<a class="B1" a href="https://2010.igem.org/Team:Peking/Project/Biosensor">Biosensor<img src="https://static.igem.org/mediawiki/2010/1/1b/Sensorintro.png"/></a>
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<a class="B2" a href="https://2010.igem.org/Team:Peking/Project/Bioabsorbent">Bioabsorbent<img src="https://static.igem.org/mediawiki/2010/8/84/Absorbentintro.png"/></a>
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<a class="B3" a href="https://2010.igem.org/Team:Peking/Project/Application">Application<img src="https://static.igem.org/mediawiki/2010/b/b6/Applicationintro.png"/></a>
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<a class="B4" a href="https://2010.igem.org/Team:Peking/Project/Expansion">Expansion<img src="https://static.igem.org/mediawiki/2010/b/b1/Expansionintro.png"/></a>
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==Parts==
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<html><a href="https://2010.igem.org/Team:Peking/Parts"><img src="https://static.igem.org/mediawiki/2010/d/da/Pku_part3.jpg" width=350 height=135></a></html>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;Our team submitted a library of thoroughly characterized and standardized parts. Therefore contributing an alternative set of tools towards heavy metal detection and decontamination in the iGEM context. What's more, as our MerR-family-derivated engineering method could be applicable in nearly all cases of heavy metal, our parts and corresponding documents provide a expansible method for heavy metal bioreporter and bioabsorbent construction.</p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <html><a href="https://2010.igem.org/Team:Peking/Parts"><font size=4>learn more</font></a></p></html>
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==Team==
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<html><a href="https://2010.igem.org/Team:Peking/Team"> <img src="https://static.igem.org/mediawiki/2010/c/c2/TeamPKUPp.jpg" width=510></a></html>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;Eighteen students and four instructors are working on our project during this summer. We split up into several subgroups whose focus and results you can follow on the Notebook or Project pages. If you want to know more about the subgroups and the people involved, meet us on our Team page.</p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<html><a href="https://2010.igem.org/Team:Peking/Team"><font size=4>learn more</font></a></p></html>
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==Modeling==
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<html><a href="https://2010.igem.org/Team:Peking/Modeling"><img src="https://static.igem.org/mediawiki/2010/7/76/Modelingpku.jpg" width=500 height=230 alt="Modeling"  ></a>
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&nbsp;&nbsp;&nbsp;&nbsp;We adopt the process of Reverse Engineering which in our work means to enumerate all possible network topologies and analysis whether they fit the objection function or not, thus getting the right topology and the proper range of parameters for the designation of our project.<br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://2010.igem.org/Team:Peking/Modeling"><font size=4>learn more</font></a>
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==Human Practice==
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<html><a href="https://2010.igem.org/Team:Peking/Humanpractice"><img src="https://static.igem.org/mediawiki/2010/8/84/FrontpageHP.jpg" width=350 height=127 alt="Biosafety"  align="right"></a>
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&nbsp;&nbsp;&nbsp;&nbsp;DNA recombination, Fragment synthesis, Bio-security... these are words that could hardly be ignored when doing researches of synthetic biology, which promises a great many improvements to our understanding of this world. The potential bio-security risk, however, is just around corner. <br>
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&nbsp;&nbsp;&nbsp;&nbsp; This summer, we did a thorough research on this topic, aiming at proactively finding out the current situation in China, and raising the awareness of researchers on such issues.<br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://2010.igem.org/Team:Peking/Humanpractice"><font size=4>learn more</font></a>
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==Gallery==
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<p>&nbsp;&nbsp;&nbsp;&nbsp;It's an unforgettable summer, during which we spared no effort to fight for our dreams and the honour of Peking iGEM team. Photos recorded every footprint of everyone. Join us together!</p>
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<html><a href="https://2010.igem.org/Team:Peking/Team/Gallery">
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<img src="https://static.igem.org/mediawiki/2010/2/2d/PKU_IGEM20102.gif" width="100px" alt="the whole team" >&nbsp;
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&nbsp;<img src="https://static.igem.org/mediawiki/2010/2/2f/%E5%9B%BE%E5%83%8F0201.jpg" width="100px" alt="Balloon on the experiment table" width="50px" >&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2010/0/03/Rats.gif" width="100px" alt="Team's mascot~" >&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2010/4/46/Assembled.jpg" width="100px" alt="Our lab">&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2010/8/88/P_large_oReI_3ff9000598fc2d11.jpg" width="115px" alt="Ao Liu & Ying Shen's birthday party">&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2010/1/1c/P_large_MA7H_33dd00001e802d0b.jpg" width="230px" alt="Weekly Meeting"></a> &nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2010/a/ab/Pkupuzzle521.jpg" width="100px" alt="Team's pyjama~"></a></html>
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<font size=5>Count Down</font><br><br>
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<font size=5>&nbsp;&nbsp;&nbsp;&nbsp;Sponsors</font><br><br>
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<html>&nbsp;&nbsp;&nbsp;&nbsp;<a href = "http://ctb.pku.edu.cn/main/en/index.htm" target="_blank"><img src="https://static.igem.org/mediawiki/2010/5/52/CTBbanner.gif" width="172px" height="50px" id="imggrey"></a>
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<a href = "http://yuanpei.pku.edu.cn/index_en.php" target="_blank"><img src="https://static.igem.org/mediawiki/2010/9/9e/YUANPEI_COLLEGE.png" width="172px" height="50px"id="imggrey"></a>
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Latest revision as of 22:17, 27 October 2010

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Parts

    Our team submitted a library of thoroughly characterized and standardized parts. Therefore contributing an alternative set of tools towards heavy metal detection and decontamination in the iGEM context. What's more, as our MerR-family-derivated engineering method could be applicable in nearly all cases of heavy metal, our parts and corresponding documents provide a expansible method for heavy metal bioreporter and bioabsorbent construction.

                                                          learn more

Team

    Eighteen students and four instructors are working on our project during this summer. We split up into several subgroups whose focus and results you can follow on the Notebook or Project pages. If you want to know more about the subgroups and the people involved, meet us on our Team page.


                                                                                                 learn more

Modeling

Modeling     We adopt the process of Reverse Engineering which in our work means to enumerate all possible network topologies and analysis whether they fit the objection function or not, thus getting the right topology and the proper range of parameters for the designation of our project.
                                                                                              learn more

Human Practice

Biosafety     DNA recombination, Fragment synthesis, Bio-security... these are words that could hardly be ignored when doing researches of synthetic biology, which promises a great many improvements to our understanding of this world. The potential bio-security risk, however, is just around corner.
     This summer, we did a thorough research on this topic, aiming at proactively finding out the current situation in China, and raising the awareness of researchers on such issues.
                                                           learn more


Count Down




Vistors From...

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