Team:METU Turkey/Results Discussion/CooA Expression and Purification
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<br> Purification with CS BL21 pTriEx: Loaded combined FPLC tubes from QS column(10/09/26) gave two close picks in CS column. First one corresponds to the CooA. The elutions of CooA are C13,C14 and C15. We combined them for further analyzes with EMSA and ITC and stored @+4. C16 AND C17 are combined to reload to CS column because they are contaminated another protein which is close to 40kDa. | <br> Purification with CS BL21 pTriEx: Loaded combined FPLC tubes from QS column(10/09/26) gave two close picks in CS column. First one corresponds to the CooA. The elutions of CooA are C13,C14 and C15. We combined them for further analyzes with EMSA and ITC and stored @+4. C16 AND C17 are combined to reload to CS column because they are contaminated another protein which is close to 40kDa. | ||
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<br> CooA protein contains heme group therefore its solution is seen yellow to orange | <br> CooA protein contains heme group therefore its solution is seen yellow to orange | ||
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Revision as of 20:58, 27 October 2010
3-CooA Expression and Purification
A.2. Expression in JM109 and BL21 with pKK vector We failed to express pKK vector in JM109. We have a little expression in BL21.
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